ArticleProceedings of the National Academy of Sciences of the United States of America2012
Gated rotation mechanism of site-specific recombination by ϕC31 integrase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 62 citations in OpenAlex.
- In vitro properties of large serine integrase hybrids derived from ϕC31 and TG1 integrases.Nucleic acids research · 2026Article
- Plasmid2MC: efficient cell-free generation of high-purity minicircle DNA for genome editing in mammalian cells.Communications biology · 2025Article
- Quantifying complexity in DNA structures with high resolution Atomic Force Microscopy.Nature communications · 2025Article
- Direct observation of subunit rotation during DNA strand exchange by serine recombinases.Nature communications · 2024Article
- Rational construction of a high-quality and high-efficiency biosynthetic system and fermentation optimization for A82846B based on combinatorial strategies in Amycolatopsis orientalis.Microbial cell factories · 2024Article
- The influence of coiled-coil motif of serine recombinase toward the directionality regulation.Biophysical journal · 2023Article
- Topological gelation of reconnecting polymers.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A mechanistic model of the BLADE platform predicts performance characteristics of 256 different synthetic DNA recombination circuits.PLoS computational biology · 2020Article
- Mechanistic modelling of tyrosine recombination reveals key parameters determining the performance of a CAR T cell switching circuit.Engineering biology · 2020Article
- Two convergent pathways of DNA knotting in replicating DNA molecules as revealed by θ-curve analysis.Nucleic acids research · 2018Article
- Single-Molecule Tethered Particle Motion: Stepwise Analyses of Site-Specific DNA Recombination.Micromachines · 2018Review
- Recombination directionality factor gp3 binds ϕC31 integrase via the zinc domain, potentially affecting the trajectory of the coiled-coil motif.Nucleic acids research · 2018Article
- Control of serine integrase recombination directionality by fusion with the directionality factor.Nucleic acids research · 2017Article
- Studies of global and local entanglements of individual protein chains using the concept of knotoids.Scientific reports · 2017Article
- Control of Recombination Directionality by the Listeria Phage A118 Protein Gp44 and the Coiled-Coil Motif of Its Serine Integrase.Journal of bacteriology · 2017Article
- Genome Integration and Excision by a New Streptomyces Bacteriophage, ϕJoe.Applied and environmental microbiology · 2017Article
- Single-molecule analysis of ϕC31 integrase-mediated site-specific recombination by tethered particle motion.Nucleic acids research · 2016Article
- Crossover-site sequence and DNA torsional stress control strand interchanges by the Bxb1 site-specific serine recombinase.Nucleic acids research · 2016Article
- The mechanism of ϕC31 integrase directionality: experimental analysis and computational modelling.Nucleic acids research · 2016Article
- Controlled rotation mechanism of DNA strand exchange by the Hin serine recombinase.Scientific reports · 2016Article
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Authors and funding
7 authors at 3 institutions in 1 country.
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Abstract
Integrases, such as that of the Streptomyces temperate bacteriophage ϕC31, promote site-specific recombination between DNA sequences in the bacteriophage and bacterial genomes to integrate or excise the phage DNA. ϕC31 integrase belongs to the serine recombinase family, a large group of structurally related enzymes with diverse biological functions. It has been proposed that serine integrases use a "subunit rotation" mechanism to exchange DNA strands after double-strand DNA cleavage at the two recombining att sites, and that many rounds of subunit rotation can occur before the strands are religated. We have analyzed the mechanism of ϕC31 integrase-mediated recombination in a topologically constrained experimental system using hybrid "phes" recombination sites, each of which comprises a ϕC31 att site positioned adjacent to a regulatory sequence recognized by Tn3 resolvase. The topologies of reaction products from circular substrates containing two phes sites support a right-handed subunit rotation mechanism for catalysis of both integrative and excisive recombination. Strand exchange usually terminates after a single round of 180° rotation. However, multiple processive "360° rotation" rounds of strand exchange can be observed, if the recombining sites have nonidentical base pairs at their centers. We propose that a regulatory "gating" mechanism normally blocks multiple rounds of strand exchange and triggers product release after a single round.
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