ArticleJournal of visualized experiments : JoVE2012
Bioluminescent bacterial imaging in vivo.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 9 citations in OpenAlex.
- Semiconducting Polymer-Based Nanocomposite for Photothermal Elimination ofMicroorganisms · 2025Article
- Validation of a Standard Luminescence Method for the Fast Determination of the Antimicrobial Activity of Nanoparticles inNanomaterials (Basel, Switzerland) · 2022Article
- Efficient protection of microorganisms for delivery to the intestinal tract by cellulose sulphate encapsulation.Microbial cell factories · 2020Article
- Options and Limitations in Clinical Investigation of Bacterial Biofilms.Clinical microbiology reviews · 2018Review
- Article
- Imaging of tumor clones with differential liver colonization.Scientific reports · 2015Article
- Tn5/7-lux: a versatile tool for the identification and capture of promoters in gram-negative bacteria.BMC microbiology · 2015Article
- Bacterial-mediated knockdown of tumor resistance to an oncolytic virus enhances therapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2014Article
- Studying interactions of Staphylococcus aureus with neutrophils by flow cytometry and time lapse microscopy.Journal of visualized experiments : JoVE · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This video describes the use of whole body bioluminesce imaging (BLI) for the study of bacterial trafficking in live mice, with an emphasis on the use of bacteria in gene and cell therapy for cancer. Bacteria present an attractive class of vector for cancer therapy, possessing a natural ability to grow preferentially within tumors following systemic administration. Bacteria engineered to express the lux gene cassette permit BLI detection of the bacteria and concurrently tumor sites. The location and levels of bacteria within tumors over time can be readily examined, visualized in two or three dimensions. The method is applicable to a wide range of bacterial species and tumor xenograft types. This article describes the protocol for analysis of bioluminescent bacteria within subcutaneous tumor bearing mice. Visualization of commensal bacteria in the Gastrointestinal tract (GIT) by BLI is also described. This powerful, and cheap, real-time imaging strategy represents an ideal method for the study of bacteria in vivo in the context of cancer research, in particular gene therapy, and infectious disease. This video outlines the procedure for studying lux-tagged E. coli in live mice, demonstrating the spatial and temporal readout achievable utilizing BLI with the IVIS system.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.