ArticleBMC biotechnology2012
Targeted DNA excision in Arabidopsis by a re-engineered homing endonuclease.
Article in BMC biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 61 citations in OpenAlex.
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- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- Tools for editing the mammalian mitochondrial genome.Human molecular genetics · 2024Review
- Methods of crop improvement and applications towards fortifying food security.Frontiers in genome editing · 2023Review
- The potential of mitochondrial genome engineering.Nature reviews. Genetics · 2022Review
- I-SceI and customized meganucleases-mediated genome editing in tomato and oilseed rape.Transgenic research · 2022Article
- Genome Editing and Designer Crops for the Future.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Mitochondrial targeted meganuclease as a platform to eliminate mutant mtDNA in vivo.Nature communications · 2021Article
- Utility of I-SceI and CCR5-ZFN nucleases in excising selectable marker genes from transgenic plants.BMC research notes · 2019Article
- Meganuclease targeting of PCSK9 in macaque liver leads to stable reduction in serum cholesterol.Nature biotechnology · 2018Article
- New breeding technique "genome editing" for crop improvement: applications, potentials and challenges.3 Biotech · 2018Review
- Engineering altered protein-DNA recognition specificity.Nucleic acids research · 2018Review
- Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity.Nucleic acids research · 2017Article
- Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses.Protein engineering, design & selection : PEDS · 2017Article
- Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.Structure (London, England : 1993) · 2016Article
- Transgenic trait deployment using designed nucleases.Plant biotechnology journal · 2016Review
- Gene stacking by recombinases.Plant biotechnology journal · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA systematic method for plant genome manipulation is a major aim of plant biotechnology. One approach to achieving this involves producing a double-strand DNA break at a genomic target site followed by the introduction or removal of DNA sequences by cellular DNA repair. Hence, a site-specific endonuclease capable of targeting double-strand breaks to unique locations in the plant genome is needed.
resultsWe engineered and tested a synthetic homing endonuclease, PB1, derived from the I-CreI endonuclease of Chlamydomonas reinhardtii, which was re-designed to recognize and cleave a newly specified DNA sequence. We demonstrate that an activity-optimized version of the PB1 endonuclease, under the control of a heat-inducible promoter, is capable of targeting DNA breaks to an introduced PB1 recognition site in the genome of Arabidopsis thaliana. We further demonstrate that this engineered endonuclease can very efficiently excise unwanted transgenic DNA, such as an herbicide resistance marker, from the genome when the marker gene is flanked by PB1 recognition sites. Interestingly, under certain conditions the repair of the DNA junctions resulted in a conservative pairing of recognition half sites to remove the intervening DNA and reconstitute a single functional recognition site.
conclusionThese results establish parameters needed to use engineered homing endonucleases for the modification of endogenous loci in plant genomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.