ArticleMicrobial cell factories2012
Enhancing solubility of deoxyxylulose phosphate pathway enzymes for microbial isoprenoid production.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 0 citations in OpenAlex.
- Integrating multimodal features with deep learning for protein solubility prediction.Journal of cheminformatics · 2026Article
- Strategic selection of microbial cell factories for sustainable and application-specific terpenoid production.Frontiers in bioengineering and biotechnology · 2026Review
- Promiscuity of an Alcohol-Dependent Hemiterpene Pathway for the In Vivo Production of a Non-Natural Alkylated Tryptophan Derivative.ACS synthetic biology · 2025Article
- Decellularization of various tissues and organs through chemical methods.Tissue & cell · 2024Review
- Charged Polypeptide Tail Boosts the Salt Resistance of Enzyme-Containing Complex Coacervate Micelles.Biomacromolecules · 2022Article
- Cellular assays identify barriers impeding iron-sulfur enzyme activity in a non-native prokaryotic host.eLife · 2022Article
- Cloning, expression, and in silico structural modeling of cholesterol oxidase of Acinetobacter sp. strain RAMD in E. coli.FEBS open bio · 2021Article
- Impact of culture condition modulation on the high-yield, high-specificity and cost-effective production of terpenoids from microbial sources: A review.Applied and environmental microbiology · 2021Review
- Aerobic acetone-butanol-isopropanol (ABI) fermentation through a co-culture ofMetabolic engineering communications · 2020Article
- Improving protein solubility and activity by introducing small peptide tags designed with machine learning models.Metabolic engineering communications · 2020Article
- Modular engineering for microbial production of carotenoids.Metabolic engineering communications · 2020Review
- Analysis of Methods to Improve the Solubility of Recombinant Bovine Sex Determining Region Y Protein.Reports of biochemistry & molecular biology · 2019Article
- Surface patches on recombinant erythropoietin predict protein solubility: engineering proteins to minimise aggregation.BMC biotechnology · 2019Article
- Challenges and Hallmarks of Establishing Alkylacetylphosphonates as Probes of Bacterial 1-Deoxy-d-xylulose 5-Phosphate Synthase.ACS infectious diseases · 2017Article
- Fermentative production and direct extraction of (-)-α-bisabolol in metabolically engineered Escherichia coli.Microbial cell factories · 2016Article
- Isoprenoid-Based Biofuels: Homologous Expression and Heterologous Expression in Prokaryotes.Applied and environmental microbiology · 2016Review
- Influence of in situ progressive N-terminal is still controversial truncation of glycogen branching enzyme in Escherichia coli DH5α on glycogen structure, accumulation, and bacterial viability.BMC microbiology · 2015Article
- Statistical experimental design guided optimization of a one-pot biphasic multienzyme total synthesis of amorpha-4,11-diene.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecombinant proteins are routinely overexpressed in metabolic engineering. It is well known that some over-expressed heterologous recombinant enzymes are insoluble with little or no enzymatic activity. This study examined the solubility of over-expressed homologous enzymes of the deoxyxylulose phosphate pathway (DXP) and the impact of inclusion body formation on metabolic engineering of microbes.
resultsFour enzymes of this pathway (DXS, ISPG, ISPH and ISPA), but not all, were highly insoluble, regardless of the expression systems used. Insoluble dxs (the committed enzyme of DXP pathway) was found to be inactive. Expressions of fusion tags did not significantly improve the solubility of dxs. However, hypertonic media containing sorbitol, an osmolyte, successfully doubled the solubility of dxs, with the concomitant improvement in microbial production of the metabolite, DXP. Similarly, sorbitol significantly improved the production of soluble and functional ERG12, the committed enzyme in the mevalonate pathway.
conclusionThis study demonstrated the unanticipated findings that some over-expressed homologous enzymes of the DXP pathway were highly insoluble, forming inclusion bodies, which affected metabolite formation. Sorbitol was found to increase both the solubility and function of some of these over-expressed enzymes, a strategy to increase the production of secondary metabolites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.