ArticleThe Journal of general physiology2012
Preferential binding of K+ ions in the selectivity filter at equilibrium explains high selectivity of K+ channels.
Article in The Journal of general physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Terahertz Modulation of Biomolecular Dynamics: Physical Mechanisms, Biological Implications, and Future Challenges.Research (Washington, D.C.) · 2026Review
- High-Throughput MicroED for Probing Ion Channel Dynamics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The Molecular Mechanism of Ion Selectivity in Nanopores.Molecules (Basel, Switzerland) · 2024Article
- Effect of THz Waves of Different Orientations on KInternational journal of molecular sciences · 2023Article
- Thermodynamics of ion binding and occupancy in potassium channels.Chemical science · 2021Article
- Structural Plasticity of the Selectivity Filter in Cation Channels.Frontiers in physiology · 2021Review
- Selectivity filter ion binding affinity determines inactivation in a potassium channel.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Modulation of Function, Structure and Clustering of KInternational journal of molecular sciences · 2020Review
- A channel profile report of the unusual KThe Journal of general physiology · 2019Article
- Queueing arrival and release mechanism for KThe journal of physiological sciences : JPS · 2019Article
- Accessibility of Cations to the Selectivity Filter of KcsA in the Inactivated State: An Equilibrium Binding Study.International journal of molecular sciences · 2019Article
- Pore size matters for potassium channel conductance.The Journal of general physiology · 2016Review
- Voltage-Gated Potassium Channels: A Structural Examination of Selectivity and Gating.Cold Spring Harbor perspectives in biology · 2016Review
- Ion-binding properties of a K+ channel selectivity filter in different conformations.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Determinants of cation transport selectivity: Equilibrium binding and transport kinetics.The Journal of general physiology · 2015Review
- Isothermal titration calorimetry of ion-coupled membrane transporters.Methods (San Diego, Calif.) · 2015Article
- Sodium and potassium competition in potassium-selective and non-selective channels.Nature communications · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
K(+) channels exhibit strong selectivity for K(+) ions over Na(+) ions based on electrophysiology experiments that measure ions competing for passage through the channel. During this conduction process, multiple ions interact within the region of the channel called the selectivity filter. Ion selectivity may arise from an equilibrium preference for K(+) ions within the selectivity filter or from a kinetic mechanism whereby Na(+) ions are precluded from entering the selectivity filter. Here, we measure the equilibrium affinity and selectivity of K(+) and Na(+) ions binding to two different K(+) channels, KcsA and MthK, using isothermal titration calorimetry. Both channels exhibit a large preference for K(+) over Na(+) ions at equilibrium, in line with electrophysiology recordings of reversal potentials and Ba(2+) block experiments used to measure the selectivity of the external-most ion-binding sites. These results suggest that the high selectivity observed during ion conduction can originate from a strong equilibrium preference for K(+) ions in the selectivity filter, and that K(+) selectivity is an intrinsic property of the filter. We hypothesize that the equilibrium preference for K(+) ions originates in part through the optimal spacing between sites to accommodate multiple K(+) ions within the selectivity filter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.