ArticleParasites & vectors2012
Population genetic analysis and sub-structuring of Theileria parva in the northern and eastern parts of Zambia.
Article in Parasites & vectors, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Tick-Borne Diseases in Sub-Saharan Africa: A Systematic Review of Pathogens, Research Focus, and Implications for Public Health.Pathogens (Basel, Switzerland) · 2024Pooled it
- Antigenic and genotypic relatedness of buffalo-derivedInternational journal for parasitology. Parasites and wildlife · 2026Article
- In vitro efficacy of plumbagin and thymol againstJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2023Article
- Epidemiology of Ticks and Tick-Borne Pathogens in Domestic Ruminants across Southern African Development Community (SADC) Region from 1980 until 2021: A Systematic Review and Meta-Analysis.Pathogens (Basel, Switzerland) · 2022Review
- Detection of Tick-Borne Bacterial and Protozoan Pathogens in Ticks from the Zambia-Angola Border.Pathogens (Basel, Switzerland) · 2022Article
- Sequence Diversity of Tp1 and Tp2 Antigens and Population Genetic Analysis ofPathogens (Basel, Switzerland) · 2022Article
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- Investigation of the piroplasm diversity circulating in wildlife and cattle of the greater Kafue ecosystem, Zambia.Parasites & vectors · 2020Article
- Sequence diversity of cytotoxic T cell antigens and satellite marker analysis of Theileria parva informs the immunization against East Coast fever in Rwanda.Parasites & vectors · 2020Article
- Genetic Diversity and Sequence Polymorphism of Two Genes Encoding Theileria parva Antigens Recognized by CD8Pathogens (Basel, Switzerland) · 2020Article
- Population Genetic Analysis of theFrontiers in microbiology · 2020Article
- Genetic and antigenic variation of the bovine tick-borne pathogen Theileria parva in the Great Lakes region of Central Africa.Parasites & vectors · 2019Article
- Population Genetic Analysis ofFrontiers in genetics · 2018Article
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- Studies of trypanosomiasis in the Luangwa valley, north-eastern Zambia.Parasites & vectors · 2015Article
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- Not "out of Nantucket": Babesia microti in southern New England comprises at least two major populations.Parasites & vectors · 2014Article
- A pan-Theileria FRET-qPCR survey for Theileria spp. in ruminants from nine provinces of China.Parasites & vectors · 2014Article
- Phylogeny and virulence divergency analyses of Toxoplasma gondii isolates from China.Parasites & vectors · 2014Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTheileriosis, caused by Theileria parva, is an economically important disease in Africa. It is a major constraint to the development of the livestock industry in some parts of eastern, central and southern Africa. In Zambia, theileriosis causes losses of up to 10,000 cattle annually.
methodsCattle blood samples were collected for genetic analysis of Theileria parva from Isoka and Petauke districts in Zambia. Microsatellite analysis was then performed on all Theileria parva positive samples for PCR using a panel of 9 microsatellite markers. Microsatellite data was analyzed using microsatellite toolkit, GenAlEx ver. 6, Fstat ver. 2.9.3.2, and LIAN computer softwares.
resultsThe combined percentage of positive samples in both districts determined by PCR using the p104 gene primers was 54.9% (95% CI: 46.7 - 63.1%, 78/142), while in each district, it was 44.8% (95% CI: 34.8 - 54.8%) and 76.1% (95% CI = 63.9 - 88.4%) for Isoka and Petauke districts, respectively. We analyzed the population genetic structure of Theileria parva from a total of 61 samples (33 from Isoka and 28 from Petauke) using a panel of 9 microsatellite markers encompassing the 4 chromosomes of Theileria parva. Wright's F index (FST = 0.178) showed significant differentiation between the Isoka and Petauke populations. Linkage disequilibrium was observed when populations from both districts were treated as a single population. When analyzed separately, linkage disequilibrium was observed in Kanyelele and Kalembe areas in Isoka district, Isoka district overall and in Petauke district. Petauke district had a higher multiplicity of infection than Isoka district.
conclusionPopulation genetic analyses of Theileria parva from Isoka and Petauke districts showed a low level of genotype exchange between the districts, but a high level of genetic diversity within each district population, implying genetic and geographic sub-structuring between the districts. The sub-structuring observed, along with the lack of panmixia in the populations, could have been due to low transmission levels at the time of sampling. However, the Isoka population was less diverse than the Petauke population.
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