ArticleJournal of pharmaceutical and biomedical analysis2013
The synthesis and characterization of a nuclear membrane affinity chromatography column for the study of human breast cancer resistant protein (BCRP) using nuclear membranes obtained from the LN-229 cells.
Article in Journal of pharmaceutical and biomedical analysis, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- High-Resolution Bioassay Profiling with Complemented Sensitivity and Resolution for Pancreatic Lipase Inhibitor Screening.Molecules (Basel, Switzerland) · 2022Article
- Selective GPR55 antagonism reduces chemoresistance in cancer cells.Pharmacological research · 2016Article
- Cancer stem cells and chemoresistance: The smartest survives the raid.Pharmacology & therapeutics · 2016Review
- Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays.Current pharmaceutical design · 2016Review
- The development of mitochondrial membrane affinity chromatography columns for the study of mitochondrial transmembrane proteins.Analytical biochemistry · 2015Article
- Multiple protein stationary phases: a review.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2014Review
- Comparative normal/failing rat myocardium cell membrane chromatographic analysis system for screening specific components that counteract doxorubicin-induced heart failure from Acontium carmichaeli.Analytical chemistry · 2014Article
- Characterization of a multiple endogenously expressed adenosine triphosphate-binding cassette transporters using nuclear and cellular membrane affinity chromatography columns.Journal of chromatography. A · 2014Article
- Identification of metabolites of propyrisulfuron in rats.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
BCRP expression has been reported in glioblastoma cell lines and clinical specimens and has been shown to be expressed both in purified nuclei and in the soluble cytoplasmic fraction. To date, the nuclear BCRP has not been characterized. Our laboratory has previously developed an online chromatographic approach for the study of binding interactions between ligands and protein, cellular membrane affinity chromatography. To this end, we have immobilized the nuclear membrane fragments onto an immobilized artificial membrane stationary phase (IAM), resulting in the nuclear membrane affinity chromatography (NMAC) column. Initial characterization was carried out on the radio flow detector, as well as the LC-MSD, using frontal displacement chromatography techniques. Etoposide, a substrate for BCRP, was initially tested, to determine the functional immobilization of BCRP. Frontal displacement experiments with multiple concentrations of etoposide were run and the binding affinity was determined to be 4.54 μM, which is in close agreement with literature. The BCRP was fully characterized on the NMAC column and this demonstrates that for the first time the nuclear membranes have been successfully immobilized.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.