ArticleMolecular microbiology2013
A yeast tRNA mutant that causes pseudohyphal growth exhibits reduced rates of CAG codon translation.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 32 citations in OpenAlex.
- Impact of Pus1 Pseudouridine Synthase on Specific Decoding Events inBiomolecules · 2020Article
- The molecular aetiology of tRNA synthetase depletion: induction of a GCN4 amino acid starvation response despite homeostatic maintenance of charged tRNA levels.Nucleic acids research · 2020Article
- Article
- Collaboration of tRNA modifications and elongation factor eEF1A in decoding and nonsense suppression.Scientific reports · 2018Article
- Generation of an arginine-tRNA-adapted Saccharomyces cerevisiae strain for effective heterologous protein expression.Current genetics · 2018Article
- Gene length as a regulator for ribosome recruitment and protein synthesis: theoretical insights.Scientific reports · 2017Article
- Article
- Increased levels of RNA oxidation enhance the reversion frequency in aging pro-apoptotic yeast mutants.Apoptosis : an international journal on programmed cell death · 2017Article
- Role of Pseudouridine Formation by Deg1 for Functionality of Two Glutamine Isoacceptor tRNAs.Biomolecules · 2017Article
- tRNA anticodon loop modifications ensure protein homeostasis and cell morphogenesis in yeast.Nucleic acids research · 2016Article
- Identification of the mRNA targets of tRNA-specific regulation using genome-wide simulation of translation.Nucleic acids research · 2016Article
- Review
- Article
- Functional importance of Ψ38 and Ψ39 in distinct tRNAs, amplified for tRNAGln(UUG) by unexpected temperature sensitivity of the s2U modification in yeast.RNA (New York, N.Y.) · 2015Article
- Loss of anticodon wobble uridine modifications affects tRNA(Lys) function and protein levels in Saccharomyces cerevisiae.PloS one · 2015Article
- Mean of the typical decoding rates: a new translation efficiency index based on the analysis of ribosome profiling data.G3 (Bethesda, Md.) · 2014Article
- Synonymous codons, ribosome speed, and eukaryotic gene expression regulation.Cellular and molecular life sciences : CMLS · 2014Review
- Translation initiation rate determines the impact of ribosome stalling on bacterial protein synthesis.The Journal of biological chemistry · 2014Article
- Ribosome recycling induces optimal translation rate at low ribosomal availability.Journal of the Royal Society, Interface · 2014Article
- Translation elongation can control translation initiation on eukaryotic mRNAs.The EMBO journal · 2014Article
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6 authors at 1 institution in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, the SUP70 gene encodes the CAG-decoding tRNA(Gln)(CUG). A mutant allele, sup70-65, induces pseudohyphal growth on rich medium, an inappropriate nitrogen starvation response. This mutant tRNA is also a UAG nonsense suppressor via first base wobble. To investigate the basis of the pseudohyphal phenotype, 10 novel sup70 UAG suppressor alleles were identified, defining positions in the tRNA(Gln)(CUG) anticodon stem that restrict first base wobble. However, none conferred pseudohyphal growth, showing altered CUG anticodon presentation cannot itself induce pseudohyphal growth. Northern blot analysis revealed the sup70-65 tRNA(Gln)(CUG) is unstable, inefficiently charged, and 80% reduced in its effective concentration. A stochastic model simulation of translation predicted compromised expression of CAG-rich ORFs in the tRNA(Gln)(CUG)-depleted sup70-65 mutant. This prediction was validated by demonstrating that luciferase expression in the mutant was 60% reduced by introducing multiple tandem CAG (but not CAA) codons into this ORF. In addition, the sup70-65 pseudohyphal phenotype was partly complemented by overexpressing CAA-decoding tRNA(Gln)(UUG), an inefficient wobble-decoder of CAG. We thus show that introducing codons decoded by a rare tRNA near the 5' end of an ORF can reduce eukaryote translational expression, and that the mutant tRNA(CUG)(Gln) constitutive pseudohyphal differentiation phenotype correlates strongly with reduced CAG decoding efficiency.
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