ArticlePLoS neglected tropical diseases2012
Sensitive and specific immunohistochemical diagnosis of human alveolar echinococcosis with the monoclonal antibody Em2G11.
Article in PLoS neglected tropical diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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49 citing papers in PubMed, 74 citations in OpenAlex.
- Article
- Small particles of Echinococcus granulosus (spegs) and Echinococcus multilocularis (spems) promote follicular T helper cell expansion and are associated with IgE and IgG4 class switching in human lymph nodes.Parasites & vectors · 2026Article
- Imaging mass cytometry of the immune microenvironment in alveolar echinococcosis.Frontiers in cellular and infection microbiology · 2026Article
- [Pathology of echinococcosis : A non-neoplastic tumour-like lesion of the liver].Pathologie (Heidelberg, Germany) · 2025Article
- The initial stage of alveolar echinococcosis is a diagnostic challenge: a case report.Journal of medical case reports · 2025Article
- Human Alveolar Echinococcosis-A Neglected Zoonotic Disease Requiring Urgent Attention.International journal of molecular sciences · 2025Review
- Advances in Novel Diagnostic Techniques for Alveolar Echinococcosis.Diagnostics (Basel, Switzerland) · 2025Review
- Diagnosis and treatment of hepatic hilar lymph node metastasis in hepatic alveolar echinococcosis patients: a real-world single-center experience.Frontiers in oncology · 2025Article
- Identification and characterization of the elusive protein backbone of the immuno-dominant and species-specific Em2(G11) metacestode antigen ofFrontiers in parasitology · 2025Article
- Fifty-five cases of hepatic alveolar echinococcosis combined with lymph node metastasis: A retrospective study.World journal of gastroenterology · 2024Article
- Immunohistochemistry as a Reliable Tool for the Diagnosis of Cystic Echinococcosis in Patients from Sardinia, Italy-A Confirmatory Study.Diseases (Basel, Switzerland) · 2024Article
- Confirmed Autochthonous Case of Human Alveolar Echinococcosis, Italy, 2023.Emerging infectious diseases · 2024Article
- Mucins Shed from the Laminated Layer in Cystic Echinococcosis Are Captured by Kupffer Cells via the Lectin Receptor Clec4F.Infection and immunity · 2023Article
- Immunology of a unique biological structure: the Echinococcus laminated layer.Protein & cell · 2023Article
- Basic Operative Tactics for Pulmonary Echinococcosis in the Era of Endostaplers and Energy Devices.Medicina (Kaunas, Lithuania) · 2023Review
- Monoclonal antibody-based localization of major diagnostic antigens in metacestode tissue, excretory/secretory products, and extracellular vesicles ofFrontiers in cellular and infection microbiology · 2023Article
- European Haplotype of <i>Echinococcus multilocularis</i> in the United States.The New England journal of medicine · 2022Article
- Stage-Oriented CT Classification and Intermodal Evolution Model in Hepatic Alveolar Echinococcosis.RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin · 2022Article
- Immunohistochemical diagnosis of human infectious diseases: a review.Diagnostic pathology · 2022Review
- Transplantation of adipose-derived stem cells ameliorates Echinococcus multilocularis-induced liver fibrosis in mice.PLoS neglected tropical diseases · 2022Article
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Authors and funding
15 authors at 7 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlveolar echinococcosis (AE) is caused by the metacestode stage of Echinococcus multilocularis. Differential diagnosis with cystic echinococcosis (CE) caused by E. granulosus and AE is challenging. We aimed at improving diagnosis of AE on paraffin sections of infected human tissue by immunohistochemical testing of a specific antibody. METHODOLOGY/PRINCIPAL
findingsWe have analysed 96 paraffin archived specimens, including 6 cutting needle biopsies and 3 fine needle aspirates, from patients with suspected AE or CE with the monoclonal antibody (mAb) Em2G11 specific for the Em2 antigen of E. multilocularis metacestodes. In human tissue, staining with mAb Em2G11 is highly specific for E. multilocularis metacestodes while no staining is detected in CE lesions. In addition, the antibody detects small particles of E. multilocularis (spems) of less than 1 µm outside the main lesion in necrotic tissue, liver sinusoids and lymphatic tissue most probably caused by shedding of parasitic material. The conventional histological diagnosis based on haematoxylin and eosin and PAS stainings were in accordance with the immunohistological diagnosis using mAb Em2G11 in 90 of 96 samples. In 6 samples conventional subtype diagnosis of echinococcosis had to be adjusted when revised by immunohistology with mAb Em2G11. CONCLUSIONS/SIGNIFICANCE: Immunohistochemistry with the mAb Em2G11 is a new, highly specific and sensitive diagnostic tool for AE. The staining of small particles of E. multilocularis (spems) outside the main lesion including immunocompetent tissue, such as lymph nodes, suggests a systemic effect on the host.
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