ArticlePloS one2012
The stability of complement-mediated bactericidal activity in human serum against Salmonella.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
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- Putative Role of an ABC Efflux System inAntibiotics (Basel, Switzerland) · 2023Article
- Snap-freezing in the Field: Effect of Sample Holding Time on Performance of Bactericidal Assays.Integrative and comparative biology · 2022Article
- Ecoimmunology in the field: Measuring multiple dimensions of immune function with minimally invasive, field-adapted techniques.American journal of human biology : the official journal of the Human Biology Council · 2022Review
- A Comparative Study of Body Lice and Bed Bugs Reveals Factors Potentially Involved in Differential Vector Competence for the Relapsing Fever Spirochete Borrelia recurrentis.Infection and immunity · 2022Article
- RND Efflux Systems Contribute to Resistance and Virulence ofAntibiotics (Basel, Switzerland) · 2021Article
- Identification of a lytic Pseudomonas aeruginosa phage depolymerase and its anti-biofilm effect and bactericidal contribution to serum.Virus genes · 2019Article
- Characterization of Clinically Relevant Strains of Extended-Spectrum β-Lactamase-ProducingFrontiers in microbiology · 2019Article
- Age-related variations in theCentral-European journal of immunology · 2018Article
- Serum bactericidal assay for the evaluation of typhoid vaccine using a semi-automated colony-counting method.Microbial pathogenesis · 2016Article
- Relationship between antibody susceptibility and lipopolysaccharide O-antigen characteristics of invasive and gastrointestinal nontyphoidal Salmonellae isolates from Kenya.PLoS neglected tropical diseases · 2015Article
- Serum bactericidal assays to evaluate typhoidal and nontyphoidal Salmonella vaccines.Clinical and vaccine immunology : CVI · 2014Article
- Polysomnographic correlates of inflammatory complement components in young healthy males.Sleep science (Sao Paulo, Brazil)Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The complement cascade includes heat-labile proteins and care is required when handling serum in order to preserve its functional integrity. We have previously used a whole human serum bactericidal assay to show that antibody and an intact complement system are required in blood for killing of invasive isolates of Salmonella. The aim of the present study was to evaluate the conditions under which human serum can be stored and manipulated while maintaining complement integrity. Serum bactericidal activity against Salmonella was maintained for a minimum of 35 days when stored at 4°C, eight days at 22°C and 54 hours at 37°C. Up to three freeze-thaw cycles had no effect on the persistence of bactericidal activity and hemolytic complement assays confirmed no effect on complement function. Delay in the separation of serum for up to four days from clotted blood stored at 22°C did not affect bactericidal activity. Dilution of serum resulted in an increased rate of loss of bactericidal activity and so serum should be stored undiluted. These findings indicate that the current guidelines concerning manipulation and storage of human serum to preserve complement integrity and function leave a large margin for safety with regards to bactericidal activity against Salmonella. The study provides a scheme for determining the requirements for serum handling in relation to functional activity of complement in other systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.