ArticlePloS one2012
An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Lipopolysaccharide-Specific Aptasensor: A Spectroscopic Approach for Identifying Gram-Negative Bacteria.Analytical science advances · 2026Article
- A Monolithic, Thiol-Functionalized Au-Based Bio-CMOS Aptasensor for Rapid, Label-Free Detection ofBioengineering (Basel, Switzerland) · 2026Article
- Biosensing technologies for foodborne pathogen detection and healthcare: principles, emerging materials, and intelligent platforms.Mikrochimica acta · 2026Review
- Electrochemical and Optical Multi-Detection ofBiosensors · 2024Article
- Highly sensitive SERS platform for pathogen analysis by cyclic DNA nanostructure@AuNP tags and cascade primer exchange reaction.Journal of nanobiotechnology · 2024Article
- Rapid and Ultrasensitive Colorimetric Biosensors for Onsite Detection ofACS sensors · 2024Article
- Aptamer-functionalized polydiacetylene biosensor for the detection of three foodborne pathogens.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Aptamer-Based Technologies for Parasite Detection.Sensors (Basel, Switzerland) · 2023Review
- Detection ofSensors (Basel, Switzerland) · 2022Article
- Aptamer-based approaches for the detection of waterborne pathogens.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- Binding Characteristics Study of DNA based Aptamers forMolecules (Basel, Switzerland) · 2021Article
- Review
- Point-of-care detection ofIranian journal of basic medical sciences · 2020Article
- Benchtop-fabricated lipid-based electrochemical sensing platform for the detection of membrane disrupting agents.Scientific reports · 2020Article
- Electrochemical sandwich immunoassay for Escherichia coli O157:H7 based on the use of magnetic nanoparticles and graphene functionalized with electrocatalytically active Au@Pt core/shell nanoparticles.Mikrochimica acta · 2018Article
- Application of Aptamer-Based Biosensor for Rapid Detection of PathogenicSensors (Basel, Switzerland) · 2018Review
- Nucleic acid aptamer-based methods for diagnosis of infections.Biosensors & bioelectronics · 2018Review
- Responsive Polydiacetylene Vesicles for Biosensing Microorganisms.Sensors (Basel, Switzerland) · 2018Review
- Biomolecule-Functionalized Smart Polydiacetylene for Biomedical and Environmental Sensing.Molecules (Basel, Switzerland) · 2018Review
- Multifunctional Nanotechnology-Enabled Sensors for Rapid Capture and Detection of Pathogens.Sensors (Basel, Switzerland) · 2017Review
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAn aptamer based biosensor (aptasensor) was developed and evaluated for rapid colorimetric detection of Escherichia coli (E. coli) O157:H7. METHODOLOGY/PRINCIPAL
findingsThe aptasensor was assembled by modifying the truncated lipopolysaccharides (LPS)-binding aptamer on the surface of nanoscale polydiacetylene (PDA) vesicle using peptide bonding between the carboxyl group of the vesicle and the amine group of the aptamer. Molecular recognition between E. coli O157:H7 and aptamer at the interface of the vesicle lead to blue-red transition of PDA which was readily visible to the naked eyes and could be quantified by colorimetric responses (CR). Confocal laser scanning microscope (CLSM) and transmission electron microscopy (TEM) was used to confirm the specific interactions between the truncated aptamer and E. coli O157:H7. The aptasensor could detect cellular concentrations in a range of 10(4)~ 10(8) colony-forming units (CFU)/ml within 2 hours and its specificity was 100% for detection of E. coli O157:H7. Compared with the standard culture method, the correspondent rate was 98.5% for the detection of E. coli O157:H7 on 203 clinical fecal specimens with our aptasensor.
conclusionsThe new aptasensor represents a significant advancement in detection capabilities based on the combination of nucleic acid aptamer with PDA vesicle, and offers a specific and convenient screening method for the detection of pathogenic bacteria. This technic could also be applied in areas from clinical analysis to biological terrorism defense, especially in low-resource settings.
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