ArticlePloS one2012
Identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.
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Who cites it
7 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.
- Serum anti-phospholipase A2 receptor (PLA2R) antibody detected at diagnosis as a predictor for clinical remission in patients with primary membranous nephropathy: a meta-analysis.BMC nephrology · 2019Pooled it
- P3H4 is correlated with clinicopathological features and prognosis in bladder cancer.World journal of surgical oncology · 2018Article
- P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.The Journal of biological chemistry · 2017Article
- Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Article
- HLA-DQA1 and PLA2R1 polymorphisms and risk of idiopathic membranous nephropathy.Clinical journal of the American Society of Nephrology : CJASN · 2014Article
- Primary glomerulonephritis: A review of important recent discoveries.Kidney research and clinical practice · 2013Review
- Collagen prolyl 3-hydroxylation: a major role for a minor post-translational modification?Connective tissue research · 2013Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membranous Nephropathy (MN) represents a large amount of Nephrotic Syndromes in the adult population and its definitive diagnosis is currently carried out through biopsy. An autoimmune condition has been demonstrated in idiopathic MN (iMN) in which some kidney structures are targeted by patient autoantibodies. Some candidate antigens have been described and other likely involved target proteins responsible for the disease are not known yet. In this work our aim is to identify these proteins by screening a lambda-phage library with patients' sera. We enrolled four groups of patients: two MN groups of 12 full iMN patients; one control group of 15 patients suffering from other renal diseases; one control group of 15 healthy individuals. A commercial cDNA phagemide library was screened using the above described sera, in order to detect positive signals due to antigen-antibody bond. We detected one phagemide clone expressing a protein which was shown to be targeted by the antibodies of the iMN sera only. Control sera were negative. The sequence analysis of cDNA matched the Synaptonemal Complex protein 65 (SC65) coding sequence. Further proteomic analyses were carried out to validate our results. We provide evidence of an involvement of SC65 protein as an autoimmune target in iMN. Considering the invasiveness and the resulting risk coming from renal biopsy, our ongoing aim is to set a procedure able to diagnose affected patients through a little- or non-invasive method such as blood sampling rather than biopsy.
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