ArticlePloS one2012
3D differentiation of neural stem cells in macroporous photopolymerizable hydrogel scaffolds.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Review
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- Promoting Alzheimer's disease research and therapy with stem cell technology.Stem cell research & therapy · 2024Review
- Impact of Modifications from Potassium Hydroxide on Porous Semi-IPN Hydrogel Properties and Its Application in Cultivation.Polymers · 2024Article
- Modern advances in spinal cord regeneration: hydrogel combined with neural stem cells.Frontiers in pharmacology · 2024Review
- Aligned Collagen Sponges with Tunable Pore Size for Skeletal Muscle Tissue Regeneration.Journal of functional biomaterials · 2023Article
- Chitosan Scaffolds as Microcarriers for Dynamic Culture of Human Neural Stem Cells.Pharmaceutics · 2023Article
- Toward a New Generation of Bio-Scaffolds for Neural Tissue Engineering: Challenges and Perspectives.Pharmaceutics · 2023Review
- Current progress of cerebral organoids for modeling Alzheimer's disease origins and mechanisms.Bioengineering & translational medicine · 2023Review
- Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.Advanced healthcare materials · 2022Article
- Fabrication Parameter-Dependent Physico-Chemical Properties of Thiolated Gelatin/PEGDA Interpenetrating Network Hydrogels.Tissue engineering and regenerative medicine · 2022Article
- 3D hydrogel models of the neurovascular unit to investigate blood-brain barrier dysfunction.Neuronal signaling · 2021Review
- Modelling of Stem Cells Microenvironment Using Carbon-Based Scaffold for Tissue Engineering Application-A Review.Polymers · 2021Review
- Innovations in 3-Dimensional Tissue Models of Human Brain Physiology and Diseases.Advanced functional materials · 2020Article
- Metabolomic and Signaling Programs Induced by Immobilized versus Soluble IFN γ in Neural Stem Cells.Bioconjugate chemistry · 2020Article
- Supermagnetic Sugarcane Bagasse Hydrochar for Enhanced Osteoconduction in Human Adipose Tissue-Derived Mesenchymal Stem Cells.Nanomaterials (Basel, Switzerland) · 2020Article
- Covalently Immobilizing Interferon-γ Drives Filopodia Production through Specific Receptor-Ligand Interactions Independently of Canonical Downstream Signaling.Bioconjugate chemistry · 2020Article
- Subcutaneous priming of protein-functionalized chitosan scaffolds improves function following spinal cord injury.Materials science & engineering. C, Materials for biological applications · 2020Article
- Using human induced pluripotent stem cells (hiPSCs) to investigate the mechanisms by which Apolipoprotein E (APOE) contributes to Alzheimer's disease (AD) risk.Neurobiology of disease · 2020Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neural stem/progenitor cells (NSPCs) are the stem cell of the adult central nervous system (CNS). These cells are able to differentiate into the major cell types found in the CNS (neurons, oligodendrocytes, astrocytes), thus NSPCs are the mechanism by which the adult CNS could potentially regenerate after injury or disorder. Microenviromental factors are critical for guiding NSPC differentiation and are thus important for neural tissue engineering. In this study, D-mannitol crystals were mixed with photocrosslinkable methacrylamide chitosan (MAC) as a porogen to enhance pore size during hydrogel formation. D-mannitol was admixed to MAC at 5, 10 and 20 wt% D-mannitol per total initial hydrogel weight. D-mannitol crystals were observed to dissolve and leave the scaffold within 1 hr. Quantification of resulting average pore sizes showed that D-mannitol addition resulted in larger average pore size (5 wt%, 4060±160 µm(2), 10 wt%, 6330±1160 µm(2), 20 wt%, 7600±1550 µm(2)) compared with controls (0 wt%, 3150±220 µm(2)). Oxygen diffusion studies demonstrated that larger average pore area resulted in enhanced oxygen diffusion through scaffolds. Finally, the differentiation responses of NSPCs to phenotypic differentiation conditions were studied for neurons, astrocytes and oligodendrocytes in hydrogels of varied porosity over 14 d. Quantification of total cell numbers at day 7 and 14, showed that cell numbers decreased with increased porosity and over the length of the culture. At day 14 immunohistochemistry quantification for primary cell types demonstrated significant differentiation to the desired cells types, and that total percentages of each cell type was greatest when scaffolds were more porous. These results suggest that larger pore sizes in MAC hydrogels effectively promote NSPC 3D differentiation.
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