ArticlePloS one2012
Identification and cluster analysis of Streptococcus pyogenes by MALDI-TOF mass spectrometry.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Performance of the matrix-assisted laser desorption ionization time-of-flight mass spectrometry system for rapid identification of streptococci: a review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Pooled it
- An emm-type specific qPCR to track bacterial load during experimental human Streptococcus pyogenes pharyngitis.BMC infectious diseases · 2021Trial
- A core-genome multilocus sequence typing scheme for the detection of genetically relatedJournal of clinical microbiology · 2023Article
- Discrimination and Characterization ofMicroorganisms · 2022Article
- Network Analysis Based on Unique Spectral Features Enables an Efficient Selection of Genomically Diverse Operational Isolation Units.Microorganisms · 2021Article
- Streptococcus suis serotyping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.PloS one · 2021Article
- Antibody Modified Gold Electrode as an Impedimetric Biosensor for the Detection ofSensors (Basel, Switzerland) · 2020Article
- Synovial Fluid Cell Proteomic Analysis Identifies Upregulation of Alpha-Taxilin Proteins in Rheumatoid Arthritis: A Potential Prognostic Marker.Journal of immunology research · 2020Article
- Rapid Classification of Multilocus Sequence Subtype for Group BFrontiers in cellular and infection microbiology · 2020Article
- Molecular Epidemiology ofInfection and drug resistance · 2020Article
- Evaluation of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry and ClinPro Tools as a Rapid Tool for TypingOpen forum infectious diseases · 2019Article
- Article
- Rapid MALDI biotyper-based identification and cluster analysis of Streptococcus iniae.Journal of microbiology (Seoul, Korea) · 2017Article
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- Identification of Streptococcus pyogenes - Phenotypic Tests vs Molecular Assay (spy1258PCR): A Comparative Study.Journal of clinical and diagnostic research : JCDR · 2016Article
- Matrix-assisted laser desorption ionization-time of flight and comparative genomic analysis of M-18 group a Streptococcus strains associated with an acute rheumatic fever outbreak in northeast Italy in 2012 and 2013.Journal of clinical microbiology · 2015Article
- Insufficient Discriminatory Power of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Dendrograms to Determine the Clonality of Multi-Drug-Resistant Acinetobacter baumannii Isolates from an Intensive Care Unit.BioMed research international · 2015Article
- Detection of extended-spectrum-β-lactamase-producing Escherichia coli ST131 and ST405 clonal groups by matrix-assisted laser desorption ionization-time of flight mass spectrometry.Journal of clinical microbiology · 2014Article
- Identification of autoantibodies against transthyretin for the screening and diagnosis of rheumatoid arthritis.PloS one · 2014Article
- Article
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Authors and funding
8 authors.
Funding
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Abstract
backgroundWhole-cell matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has been successfully applied for bacterial identification and typing of many pathogens. The fast and reliable qualities of MALDI-TOF MS make it suitable for clinical diagnostics. MALDI-TOF MS for the identification and cluster analysis of Streptococcus pyogenes, however, has not been reported. The goal of our study was to evaluate this approach for the rapid identification and typing of S. pyogenes.
methods65 S. pyogenes isolates were obtained from the hospital. The samples were prepared and MALDI-TOF MS measurements were conducted as previously reported. Identification of unknown spectra was performed via a pattern recognition algorithm with a reference spectra and a dendrogram was constructed using the statistical toolbox in Matlab 7.1 integrated in the MALDI Biotyper 2.0 software.
resultsFor identification, 61 of 65 S. pyogenes isolates could be identified correctly by MALDI-TOF MS with BioType 2.0 when compared to biochemical identification (API Strep), with an accuracy of 93.85%. In clustering analysis, 44 of 65 isolates were in accordance with those established by M typing, with a matching rate of 67.69%. When only the M type prevalence in China was considered, 41 of 45 isolates were in agreement with M typing, with a matching rate of 91.1%.
conclusionsIt was here shown that MALDI-TOF MS with Soft Biotype 2.0 and its database could facilitate rapid identification of S. pyogenes. It may present an attractive alternative to traditional biochemical methods of identification. However, for classification, more isolates and advances in the MALDI-TOF MS technology are needed to improve accuracy.
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