ArticlePloS one2012
BeeDoctor, a versatile MLPA-based diagnostic tool for screening bee viruses.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 67 citations in OpenAlex.
- Deformed wing virus affects foraging success and foraging specialization of honeybee workers.Scientific reports · 2025Article
- New insights into honey bee viral and bacterial seasonal infection patterns using third-generation nanopore sequencing on honey bee haemolymph.Veterinary research · 2024Article
- Known and novel viruses in Belgian honey bees: yearly differences, spatial clustering, and associations with overwintering loss.Microbiology spectrum · 2024Article
- Viral communities in the parasite Varroa destructor and in colonies of their honey bee host (Apis mellifera) in New Zealand.Scientific reports · 2022Article
- Reduced nest development of reared Bombus terrestris within apiary dense human-modified landscapes.Scientific reports · 2021Article
- Current Status of Loop-Mediated Isothermal Amplification Technologies for the Detection of Honey Bee Pathogens.Frontiers in veterinary science · 2021Review
- Metagenomic Approach with the NetoVIR Enrichment Protocol Reveals Virus Diversity within Ethiopian Honey Bees (Viruses · 2020Article
- Article
- Improving bee health through genomics.Nature reviews. Genetics · 2020Review
- Codon optimization, expression inPeerJ · 2020Article
- Virus and dsRNA-triggered transcriptional responses reveal key components of honey bee antiviral defense.Scientific reports · 2017Article
- The virulent, emerging genotype B of Deformed wing virus is closely linked to overwinter honeybee worker loss.Scientific reports · 2017Article
- Covert deformed wing virus infections have long-term deleterious effects on honeybee foraging and survival.Proceedings. Biological sciences · 2017Article
- The Status of Honey Bee Health in Italy: Results from the Nationwide Bee Monitoring Network.PloS one · 2016Article
- A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium.Applied microbiology and biotechnology · 2015Article
- A sting in the spit: widespread cross-infection of multiple RNA viruses across wild and managed bees.The Journal of animal ecology · 2015Article
- Vertical transmission of honey bee viruses in a Belgian queen breeding program.BMC veterinary research · 2015Article
- Parasites and Pathogens of the Honeybee (Apis mellifera) and Their Influence on Inter-Colonial Transmission.PloS one · 2015Article
- Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.BioMed research international · 2015Article
- First detection of viruses in africanized honey bees from Peru.Virologica Sinica · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The long-term decline of managed honeybee hives in the world has drawn significant attention to the scientific community and bee-keeping industry. A high pathogen load is believed to play a crucial role in this phenomenon, with the bee viruses being key players. Most of the currently characterized honeybee viruses (around twenty) are positive stranded RNA viruses. Techniques based on RNA signatures are widely used to determine the viral load in honeybee colonies. High throughput screening for viral loads necessitates the development of a multiplex polymerase chain reaction approach in which different viruses can be targeted simultaneously. A new multiparameter assay, called "BeeDoctor", was developed based on multiplex-ligation probe dependent amplification (MLPA) technology. This assay detects 10 honeybee viruses in one reaction. "BeeDoctor" is also able to screen selectively for either the positive strand of the targeted RNA bee viruses or the negative strand, which is indicative for active viral replication. Due to its sensitivity and specificity, the MLPA assay is a useful tool for rapid diagnosis, pathogen characterization, and epidemiology of viruses in honeybee populations. "BeeDoctor" was used for screening 363 samples from apiaries located throughout Flanders; the northern half of Belgium. Using the "BeeDoctor", virus infections were detected in almost eighty percent of the colonies, with deformed wing virus by far the most frequently detected virus and multiple virus infections were found in 26 percent of the colonies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.