ArticlePLoS pathogens2012
Arabidopsis Actin-Depolymerizing Factor-4 links pathogen perception, defense activation and transcription to cytoskeletal dynamics.
Article in PLoS pathogens, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 108 citations in OpenAlex.
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- Phosphorylation of Plant Ferredoxin-like Protein Is Required for Intensifying PAMP-Triggered Immunity inPlants (Basel, Switzerland) · 2025Article
- A Ralstonia solanacearum type III effector alters the actin and microtubule cytoskeleton to promote bacterial virulence in plants.PLoS pathogens · 2024Article
- Logistics of defense: The contribution of endomembranes to plant innate immunity.The Journal of cell biology · 2024Review
- ZmADF5, a Maize Actin-Depolymerizing Factor Conferring Enhanced Drought Tolerance in Maize.Plants (Basel, Switzerland) · 2024Article
- Actin-Depolymerizing Factor Gene Family Analysis Revealed ThatPlants (Basel, Switzerland) · 2023Article
- Nuclear effectors of plant pathogens: Distinct strategies to be one step ahead.Molecular plant pathology · 2023Review
- VILLIN2 regulates cotton defense against Verticillium dahliae by modulating actin cytoskeleton remodeling.Plant physiology · 2023Article
- Research progress on the roles of actin-depolymerizing factor in plant stress responses.Frontiers in plant science · 2023Review
- Genome-wide identification of actin-depolymerizing factor gene family and their expression patterns under various abiotic stresses in soybean (Frontiers in plant science · 2023Article
- The Cytoskeleton in Plant Immunity: Dynamics, Regulation, and Function.International journal of molecular sciences · 2022Review
- Article
- Actin depolymerizing factor ADF7 inhibits actin bundling protein VILLIN1 to regulate root hair formation in response to osmotic stress in Arabidopsis.PLoS genetics · 2022Article
- Research on the Molecular Interaction Mechanism between Plants and Pathogenic Fungi.International journal of molecular sciences · 2022Review
- Article
- Controlling the Gate: The Functions of the Cytoskeleton in Stomatal Movement.Frontiers in plant science · 2022Review
- GhADF6-mediated actin reorganization is associated with defence against Verticillium dahliae infection in cotton.Molecular plant pathology · 2021Article
- MPK3- and MPK6-mediated VLN3 phosphorylation regulates actin dynamics during stomatal immunity in Arabidopsis.Nature communications · 2021Article
- Arabidopsis ADF5 Acts as a Downstream Target Gene of CBFs in Response to Low-Temperature Stress.Frontiers in cell and developmental biology · 2021Article
- Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity.Nature communications · 2020Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The primary role of Actin-Depolymerizing Factors (ADFs) is to sever filamentous actin, generating pointed ends, which in turn are incorporated into newly formed filaments, thus supporting stochastic actin dynamics. Arabidopsis ADF4 was recently shown to be required for the activation of resistance in Arabidopsis following infection with the phytopathogenic bacterium Pseudomonas syringae pv. tomato DC3000 (Pst) expressing the effector protein AvrPphB. Herein, we demonstrate that the expression of RPS5, the cognate resistance protein of AvrPphB, was dramatically reduced in the adf4 mutant, suggesting a link between actin cytoskeletal dynamics and the transcriptional regulation of R-protein activation. By examining the PTI (PAMP Triggered Immunity) response in the adf4 mutant when challenged with Pst expressing AvrPphB, we observed a significant reduction in the expression of the PTI-specific target gene FRK1 (Flg22-Induced Receptor Kinase 1). These data are in agreement with recent observations demonstrating a requirement for RPS5 in PTI-signaling in the presence of AvrPphB. Furthermore, MAPK (Mitogen-Activated Protein Kinase)-signaling was significantly reduced in the adf4 mutant, while no such reduction was observed in the rps5-1 point mutation under similar conditions. Isoelectric focusing confirmed phosphorylation of ADF4 at serine-6, and additional in planta analyses of ADF4's role in immune signaling demonstrates that nuclear localization is phosphorylation independent, while localization to the actin cytoskeleton is linked to ADF4 phosphorylation. Taken together, these data suggest a novel role for ADF4 in controlling gene-for-gene resistance activation, as well as MAPK-signaling, via the coordinated regulation of actin cytoskeletal dynamics and R-gene transcription.
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