ArticleJournal of bacteriology2013
FliW and FliS function independently to control cytoplasmic flagellin levels in Bacillus subtilis.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 56 citations in OpenAlex.
- Functional analysis of natural variation in the RNA-binding protein CsrA across the bacterial domain predicts regulatory activity.Nucleic acids research · 2026Article
- Mfd interacts with RNA polymerase to modulate flagellum-dependent motility, chemotaxis, and population heterogeneity in the stationary phase ofJournal of bacteriology · 2026Article
- The Flagellin-Specific Chaperone FliS of Borrelia burgdorferi Controls the Cytoplasmic Pool of Flagellins at the Level of Translation Initiation, Secretion, and Proteolysis.Molecular microbiology · 2025Article
- Swarm-seq Reveals Functional Divergence in CsrA Homologs Across Bacteria.bioRxiv : the preprint server for biology · 2025Article
- FlaG competes with FliS-flagellin complexes for access to FlhA in the flagellar T3SS to controlProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The catE gene of Bacillus licheniformis M2-7 is essential for growth in benzopyrene, and its expression is regulated by the Csr system.World journal of microbiology & biotechnology · 2023Article
- Article
- Identification of Genes Required for Swarming Motility inJournal of bacteriology · 2022Article
- Bending stiffness characterization of Bacillus subtilis' flagellar filament.Biophysical journal · 2022Article
- PncsHub: a platform for annotating and analyzing non-classically secreted proteins in Gram-positive bacteria.Nucleic acids research · 2022Article
- Microbial hitchhiking: how Streptomyces spores are transported by motile soil bacteria.The ISME journal · 2021Article
- ComEB protein is dispensable for the transformation but must be translated for the optimal synthesis of comEC.Molecular microbiology · 2021Article
- Glycosyltransferase Jhp0106 (PseE) contributes to flagellin maturation in Helicobacter pylori.Helicobacter · 2021Article
- FliW and CsrA Govern Flagellin (FliC) Synthesis and Play Pleiotropic Roles in Virulence and Physiology ofFrontiers in microbiology · 2021Article
- Binding ofFrontiers in microbiology · 2020Article
- Article
- Analysis of a flagellar filament cap mutant reveals that HtrA serine protease degrades unfolded flagellin protein in the periplasm of Borrelia burgdorferi.Molecular microbiology · 2019Article
- Article
- YfmK is an NProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Isolate Specific Cold Response ofFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The cytoplasmic level of flagellin (called Hag) is homeostatically regulated in the Gram-positive bacterium Bacillus subtilis by a partner-switching mechanism between the protein FliW and either the Hag structural protein or CsrA, an RNA binding protein that represses hag translation. Here we show that FliW and the putative secretion chaperone FliS bind to Hag simultaneously but control Hag translation by different mechanisms. While FliW directly inhibits CsrA activity, FliS antagonizes CsrA indirectly by binding to Hag, enhancing Hag secretion, and depleting Hag in the cytoplasm to trigger the FliW partner switch. Consistent with a role for FliS in potentiating Hag secretion, the mutation of fliS crippled both motility and flagellar filament assembly, and both phenotypes could be partially rescued by artificially increasing the concentration of the Hag substrate through the absence of CsrA. Furthermore, the absence of FliS resulted in an approximately 30-fold reduction in extracellular Hag accumulation in cells mutated for CsrA (to relieve homeostatic control) and the filament cap protein FliD (to secrete flagellin into the supernatant). Thus, we mechanistically discriminate between the FliW regulator and the FliS chaperone to show that secretion disrupts flagellin homeostasis and promotes high-level flagellin synthesis during the period of filament assembly in B. subtilis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.