ArticleApplied and environmental microbiology2013
Heterologous carotenoid-biosynthetic enzymes: functional complementation and effects on carotenoid profiles in Escherichia coli.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 44 citations in OpenAlex.
- Metabolic unlocking of lycopene accumulation in the oleaginous yeast Rhodotorula toruloides.Biotechnology letters · 2026Article
- MarineMarine life science & technology · 2025Article
- Genomic Insights into the Role of cAMP in Carotenoid Biosynthesis: Enhancing β-Carotene Production inInternational journal of molecular sciences · 2024Article
- Complete microbial synthesis of crocetin and crocins from glycerol in Escherichia coli.Microbial cell factories · 2024Article
- Pantoea ananatis carotenoid production confers toxoflavin tolerance and is regulated by Hfq-controlled quorum sensing.MicrobiologyOpen · 2021Article
- Genome Mining Reveals Two Missing CrtP and AldH Enzymes in the C30 Carotenoid Biosynthesis Pathway inMolecules (Basel, Switzerland) · 2020Article
- Multiplicity of carotene patterns derives from competition between phytoene desaturase diversification and biological environments.Scientific reports · 2020Article
- Microbial Production of Retinyl Palmitate and Its Application as a Cosmeceutical.Antioxidants (Basel, Switzerland) · 2020Article
- Rhodoxanthin synthase from honeysuckle; a membrane diiron enzyme catalyzes the multistep conversation of β-carotene to rhodoxanthin.Science advances · 2020Article
- Exploring natural biodiversity to expand access to microbial terpene synthesis.Microbial cell factories · 2019Article
- Generation of structurally novel short carotenoids and study of their biological activity.Scientific reports · 2016Article
- The Phytoene synthase gene family of apple (Malus x domestica) and its role in controlling fruit carotenoid content.BMC plant biology · 2015Article
- Bioinformatic analyses of integral membrane transport proteins encoded within the genome of the planctomycetes species, Rhodopirellula baltica.Biochimica et biophysica acta · 2014Article
- New insight into the cleavage reaction of Nostoc sp. strain PCC 7120 carotenoid cleavage dioxygenase in natural and nonnatural carotenoids.Applied and environmental microbiology · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A limited number of carotenoid pathway genes from microbial sources have been studied for analyzing the pathway complementation in the heterologous host Escherichia coli. In order to systematically investigate the functionality of carotenoid pathway enzymes in E. coli, the pathway genes of carotenogenic microorganisms (Brevibacterium linens, Corynebacterium glutamicum, Rhodobacter sphaeroides, Rhodobacter capsulatus, Rhodopirellula baltica, and Pantoea ananatis) were modified to form synthetic expression modules and then were complemented with Pantoea agglomerans pathway enzymes (CrtE, CrtB, CrtI, CrtY, and CrtZ). The carotenogenic pathway enzymes in the synthetic modules showed unusual activities when complemented with E. coli. For example, the expression of heterologous CrtEs of B. linens, C. glutamicum, and R. baltica influenced P. agglomerans CrtI to convert its substrate phytoene into a rare product-3,4,3',4'-tetradehydrolycopene-along with lycopene, which was an expected product, indicating that CrtE, the first enzyme in the carotenoid biosynthesis pathway, can influence carotenoid profiles. In addition, CrtIs of R. sphaeroides and R. capsulatus converted phytoene into an unusual lycopene as well as into neurosporene. Thus, this study shows that the functional complementation of pathway enzymes from different sources is a useful methodology for diversifying biosynthesis as nature does.
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