ArticlePlanta2013
Tonoplast of Beta vulgaris L. contains detergent-resistant membrane microdomains.
Article in Planta, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The trial behind it
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Who cites it
12 citing papers in PubMed, 42 citations in OpenAlex.
- Detergent-Resistant Membranes in Chloroplasts and Mitochondria of the HalophytePlants (Basel, Switzerland) · 2023Article
- Article
- Role of Plasmalemma Microdomains (Rafts) in Protection of the Plant Cell Under Osmotic Stress.The Journal of membrane biology · 2021Article
- Plasma and vacuolar membrane sphingolipidomes: composition and insights on the role of main molecular species.Plant physiology · 2021Article
- Non-detergent Isolation of Membrane Structures from Beet Plasmalemma and Tonoplast Having Lipid Composition Characteristic of Rafts.The Journal of membrane biology · 2020Article
- Article
- Sucrose Starvation Induces Microautophagy in Plant Root Cells.Frontiers in plant science · 2019Article
- Detection of lipid-protein microdomains (rafts) and investigation of their functional role in the chloroplast membranes of halophytes.Doklady. Biochemistry and biophysics · 2017Article
- Nitrate Reductase Knockout Uncouples Nitrate Transport from Nitrate Assimilation and Drives Repartitioning of Carbon Flux in a Model Pennate Diatom.The Plant cell · 2017Article
- Molecular Composition of Plant Vacuoles: Important but Less Understood Regulations and Roles of Tonoplast Lipids.Plants (Basel, Switzerland) · 2015Review
- Direct purification of detergent-insoluble membranes from Medicago truncatula root microsomes: comparison between floatation and sedimentation.BMC plant biology · 2014Article
- The endoplasmic reticulum is the main membrane source for biogenesis of the lytic vacuole in Arabidopsis.The Plant cell · 2013Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The experiments conducted on tonoplast of Beta vulgaris L. roots were performed to identify detergent-resistant lipid-protein microdomains (DRMs, interpreted as lipid rafts).The presence of DRMs can be found when dynamic clustering of sphingolipids, sterols, saturated fatty acids is registered, and the insolubility of these microdomains in nonionic detergents at low temperatures is proven. The elucidation of tonoplast microdomains has been based on results obtained with the aid of high-speed centrifuging in the sucrose gradient. The experiments have shown that tonoplast microdomains are rich in sphingolipids, free sterols and saturated fatty acids (such a lipid content is also typical of lipid-protein microdomains of other membranes), while only few phospholipids are present in tonoplast microdomains. The presence of microdomains has been confirmed by fluorescence and confocal microscopy using filipin and Laurdan as fluorescent probes. The experiments with Laurdan have shown that tonoplast microdomains are characterized by a high order compared to characteristics of the rest of the tonoplast. Thus, the presence of detergent-resistant lipid-protein microdomains in the tonoplast has been demonstrated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.