ArticleNucleic acids research2013
Mechanism of promoter repression by Lac repressor-DNA loops.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 61 citations in OpenAlex.
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- High-Resolution Characterization of DNA/Protein Complexes in Living Bacteria.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Quantitative Determination of DNA Bridging Efficiency of Chromatin Proteins.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Deep flanking sequence engineering for efficient promoter design using DeepSEED.Nature communications · 2023Article
- Positive supercoiling favors transcription elongation through lac repressor-mediated DNA loops.Nucleic acids research · 2022Article
- An operator-based expression toolkit forProceedings of the National Academy of Sciences of the United States of America · 2022Article
- DNA looping by protamine follows a nonuniform spatial distribution.Biophysical journal · 2021Article
- Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systems.Nature communications · 2021Article
- Characterization of Gene Repression by Designed Transcription Activator-like Effector Dimer Proteins.Biophysical journal · 2020Article
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- Homolog comparisons further reconcile in vitro and in vivo correlations of protein activities by revealing over-looked physiological factors.Protein science : a publication of the Protein Society · 2019Article
- Identification of a basal system for unwinding a bacterial chromosome origin.The EMBO journal · 2019Article
- TheJournal of bacteriology · 2019Article
- Bacterial gene control by DNA looping using engineered dimeric transcription activator like effector (TALE) proteins.Nucleic acids research · 2018Article
- Protein-mediated looping of DNA under tension requires supercoiling.Nucleic acids research · 2018Article
- Tailor-made transcriptional biosensors for optimizing microbial cell factories.Journal of industrial microbiology & biotechnology · 2017Review
- Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects.Biophysical reviews · 2016Review
Corrections and comments
- Erratum issuedLionberger, Troy A [added]
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The Escherichia coli lactose (lac) operon encodes the first genetic switch to be discovered, and lac remains a paradigm for studying negative and positive control of gene expression. Negative control is believed to involve competition of RNA polymerase and Lac repressor for overlapping binding sites. Contributions to the local Lac repressor concentration come from free repressor and repressor delivered to the operator from remote auxiliary operators by DNA looping. Long-standing questions persist concerning the actual role of DNA looping in the mechanism of promoter repression. Here, we use experiments in living bacteria to resolve four of these questions. We show that the distance dependence of repression enhancement is comparable for upstream and downstream auxiliary operators, confirming the hypothesis that repressor concentration increase is the principal mechanism of repression loops. We find that as few as four turns of DNA can be constrained in a stable loop by Lac repressor. We show that RNA polymerase is not trapped at repressed promoters. Finally, we show that constraining a promoter in a tight DNA loop is sufficient for repression even when promoter and operator do not overlap.
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