ArticleNucleic acids research2013
Genetic interactions suggest multiple distinct roles of the arch and core helicase domains of Mtr4 in Rrp6 and exosome function.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Post-transcriptional regulation shapes the transcriptome of quiescent budding yeast.Nucleic acids research · 2024Article
- Conserved Residues at the Mtr4 C-Terminus Coordinate Helicase Activity and Exosome Interactions.Biochemistry · 2024Article
- A yeast model for trichohepatoenteric syndrome suggests strong loss of Ski2 function in most causative mutations.microPublication biology · 2022Article
- Interplay of the RNA Exosome Complex and RNA-Binding Protein Ssd1 in Maintaining Cell Wall Stability in Yeast.Microbiology spectrum · 2021Article
- Yeast RNA exosome activity is necessary for maintaining cell wall stability through proper protein glycosylation.Molecular biology of the cell · 2021Article
- Substrate specificity of the TRAMP nuclear surveillance complexes.Nature communications · 2020Article
- Structural insights into the interaction of the nuclear exosome helicase Mtr4 with the preribosomal protein Nop53.RNA (New York, N.Y.) · 2017Article
- The Rrp6 C-terminal domain binds RNA and activates the nuclear RNA exosome.Nucleic acids research · 2017Article
- Interaction between the RNA-dependent ATPase and poly(A) polymerase subunits of the TRAMP complex is mediated by short peptides and important for snoRNA processing.Nucleic acids research · 2015Article
- The Mtr4 ratchet helix and arch domain both function to promote RNA unwinding.Nucleic acids research · 2014Article
- The exosome-binding factors Rrp6 and Rrp47 form a composite surface for recruiting the Mtr4 helicase.The EMBO journal · 2014Article
- Integrity of SRP RNA is ensured by La and the nuclear RNA quality control machinery.Nucleic acids research · 2014Article
- Threading the barrel of the RNA exosome.Trends in biochemical sciences · 2013Review
- mRNA quality control pathways in Saccharomyces cerevisiae.Journal of biosciences · 2013Review
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2 authors.
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Abstract
The RNA exosome is responsible for a wide variety of RNA processing and degradation reactions. The activity and specificity of the RNA exosome is thought to be controlled by a number of cofactors. Mtr4 is an essential RNA-dependent adenosine triphosphatase that is required for all of the nuclear functions of the RNA exosome. The crystal structure of Mtr4 uncovered a domain that is conserved in the RNA exosome cofactors Mtr4 and Ski2 but not in other helicases, suggesting it has an important role related to exosome activation. Rrp6 provides the nuclear exosome with one of its three nuclease activities, and previous findings suggested that the arch domain is specifically required for Rrp6 functions. Here, we report that the genetic interactions between the arch domain of Mtr4 and Rrp6 cannot be explained by the arch domain solely acting in Rrp6-dependent processing reactions. Specifically, we show that the arch domain is not required for all Rrp6 functions, and that the arch domain also functions independently of Rrp6. Finally, we show that the arch domain of Ski2, the cytoplasmic counterpart of Mtr4, is required for Ski2's function, thereby confirming that the arch domains of these cofactors function independently of Rrp6.
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