ArticleChild's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery2013
Changes in the biological characteristics of glioma cancer stem cells after serial in vivo subtransplantation.
Article in Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 10 citations in OpenAlex.
- Heterotypic mouse models of canine osteosarcoma recapitulate tumor heterogeneity and biological behavior.Disease models & mechanisms · 2016Article
- Failure of a patient-derived xenograft for brain tumor model prepared by implantation of tissue fragments.Cancer cell international · 2016Article
- Success of tumorsphere isolation from WHO grade IV gliomas does not correlate with the weight of fresh tumor specimens: an immunohistochemical characterization of tumorsphere differentiation.Cancer cell international · 2016Article
- Prognostic value of glioma cancer stem cell isolation in survival of primary glioblastoma patients.Stem cells international · 2014Article
- Isolation of tumor spheres and mesenchymal stem-like cells from a single primitive neuroectodermal tumor specimen.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2013Article
- Existence of glioma stroma mesenchymal stemlike cells in Korean glioma specimens.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2013Article
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15 authors at 6 institutions in 2 countries.
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Abstract
purposeCurrently, the interaction between the niche and glioma cancer stem cells (gCSCs) is gaining attention. However, there are few studies concerned with the effects of repeated exposure to a new microenvironment on gCSCs characteristics. In this study, serial in vivo subtransplantation was performed to create a new microenvironment. We evaluated and compared the biological characteristics of gCSCs after serial in vivo subtransplantation.
methodsWe cultured gCSCs from human glioma specimens according to cultured gliomasphere methods. The isolated gCSCs were termed zero-generation gCSCs (G0-gCSCs). By subsequent serial subtransplantation, we obtained first-generation gCSCs (G1-gCSCs) and second-generation gCSCs (G2-gCSCs). We evaluated and compared the biological characteristics of G0-gCSCs, G1-gCSCs, and G2-gCSCs. The in vitro characteristics included the morphology, surface marker profiles, and neural differentiation capacity and the in vivo characteristics was the survival of mice xenografts. Additionally, brain sections were analyzed using PCNA, TUNEL, and CD31 staining.
resultsWe observed no significant differences in the in vitro characteristics of G0-gCSCs, G1-gCSCs, and G2-gCSCs. However, the survival time of mice glioma xenografts was significantly decreased upon serial subtransplantation. In addition, immunohistochemical analyses showed that the number of TUNEL(+) cells was significantly decreased while the number of CD31(+) cells was significantly increased with serial in vivo subtransplantation.
conclusionsThere were significant in vivo biological changes in gCSCs upon serial in vivo subtransplantation, which were shorter xenograft survival, increased angiogenesis, and decreased apoptosis. This study suggests that the repeated exposure to new microenvironments may affect the biological changes in gCSCs in vivo.
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