Evidence map›Paper›PMID 23142810›Full record

ArticleEndocrinology2013

WNT4 acts downstream of BMP2 and functions via β-catenin signaling pathway to regulate human endometrial stromal cell differentiation.

Quanxi Li, Athilakshmi Kannan, Amrita Das, Franco J Demayo, Peter J Hornsby, Steven L Young, Robert N Taylor, Milan K Bagchi, Indrani C Bagchi

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.

0numbers the graph read from it
0cells of the map it votes in
79citing papers in PubMed
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

79 citing papers in PubMed, 126 citations in OpenAlex.

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  9. Progesterone receptor in uterine glands is required for pregnancy establishment in mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025
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19 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Quanxi LiDepartment of Comparative Biosciences, University of Illinois at Urbana-Champaign, IL 61802, USA.
Athilakshmi Kannan
Amrita Das
Franco J Demayo
Peter J Hornsby
Steven L Young
Robert N Taylor
Milan K Bagchi
Indrani C Bagchi
University of Illinois Urbana-Champaign · USBaylor College of Medicine · USThe University of Texas Health Science Center at San Antonio · USUniversity of North Carolina at Chapel Hill · USWake Forest University · US

Funding

Role of C/EBPb in Uterine Decidualization and ImplantationU54HD055787 · NICHD · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI BAGCHI, MILAN K · 2008 to 2012
$6.8M
Progesterone Action in the Endometrium of Women with EndometriosisR01HD067721 · NICHD · UNIV OF NORTH CAROLINA CHAPEL HILL · PI YOUNG, STEVEN L · 2011 to 2015
$3.1M
NICHD NIH HHS U54 HD055787
6 · The paper itself

Abstract

Differentiation of endometrial stromal cells into decidual cells is a prerequisite for successful embryo implantation. Our previous studies in the mouse have shown that bone morphogenetic protein 2 (BMP2), a morphogen belonging to the TGFβ superfamily, is essential for this differentiation process. BMP2 is markedly induced in human primary endometrial stromal cells (HESCs) as they undergo differentiation in response to steroid hormones and cAMP. The present study was undertaken to identify the BMP2-mediated molecular pathways in primary cultures of HESCs during decidualization. Using gene expression profiling, we identified wingless-related murine mammary tumor virus integration site 4 (WNT4) as a target of BMP2 regulation during decidualization. Attenuation of WNT4 expression in HESCs by small interfering RNA administration greatly reduced BMP2-induced stromal differentiation. Additionally, adenovirus-mediated overexpression of WNT4 in HESCs markedly advanced the differentiation program, indicating that it is a key regulator of decidualization. The stimulatory effect of WNT4 was accompanied by the accumulation of active β-catenin in the nuclei of decidualizing stromal cells, indicating the involvement of the canonical WNT signaling pathway. Functional inhibition of WNT4/β-catenin pathway by Dickkopf-1, an inhibitor of the canonical WNT signaling, or small interfering RNA-mediated silencing of β-catenin expression, greatly reduced the BMP2- and WNT4-induced decidualization. Gene expression profiling revealed that Forkhead box protein O1, a forkhead family transcription factor and previously reported regulator of HESC differentiation, is a common downstream mediator of both BMP2 and WNT4 signaling. Taken together, these studies uncovered a linear pathway involving BMP2, WNT4/β-catenin, and Forkhead box protein O1 that operates in human endometrium to critically control decidualization.

Indexed as

beta CateninBone Morphogenetic Protein 2Cell DifferentiationCells, CulturedEndometriumFemaleForkhead Transcription FactorsHumansRNA, Small InterferingSignal TransductionStromal CellsWnt4 Proteinbeta CateninBone Morphogenetic Protein 2Forkhead Transcription FactorsRNA, Small InterferingWnt4 ProteinWNT4 protein, human

Identifiers

PMID23142810
PMCPMC3529366
OpenAlexW1982579962

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.