ArticleToxicology and applied pharmacology2013
Cell-specific oxidative stress and cytotoxicity after wildfire coarse particulate matter instillation into mouse lung.
Article in Toxicology and applied pharmacology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Non-accidental health impacts of wildfire smoke.International journal of environmental research and public health · 2014Pooled it
- Long-term exposure to wildfire-related PMThe Lancet. Oncology · 2026Article
- Cardiopulmonary hospitalization risks from wildfire-specific and non-wildfire PMNature communications · 2026Article
- Atmospheric stressors and kidney diseases.Nature reviews. Nephrology · 2026Review
- Differential metabolic profiles by training fire exposure in female firefighters.International journal of hygiene and environmental health · 2026Article
- Wildfires and Atopic Diseases: A Review.Allergy · 2026Review
- Impact of Wildfire Smoke on Respiratory Disease Associated Healthcare Utilization in Gang-Won Province, South Korea, in 2017.GeoHealth · 2026Article
- Effects of urban PM2.5 on primary sinonasal epithelial cells in individuals with chronic rhinosinusitis.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- Wildfire Exposure and Respiratory Health: A Comprehensive Review of Emerging Evidence.Tuberculosis and respiratory diseases · 2025Article
- Long-Term Wildfire Smoke Exposure and Increased Risk of Heart Failure in Older Adults.Journal of the American College of Cardiology · 2025Article
- The 2023 Canadian Wildfires and Risk of Hospitalization and Mortality Among Hemodialysis Patients in the United States.Kidney international reports · 2025Article
- Impact of Canadian Wildfire-Emitted Particulate Matter on THP-1 Lung Macrophage Health and Function.Environmental science & technology · 2025Article
- Mortality attributable to PMScience advances · 2024Article
- Excess prenatal loss and respiratory illnesses of infant macaques living outdoors and exposed to wildfire smoke.American journal of primatology · 2024Article
- Clearing the Air: Understanding the Impact of Wildfire Smoke on Asthma and COPD.Healthcare (Basel, Switzerland) · 2024Review
- "Air That Once Was Breath" Part 1: Wildfire-Smoke-Induced Mechanisms of Airway Inflammation - "Climate Change, Allergy and Immunology" Special IAAI Article Collection: Collegium Internationale Allergologicum Update 2023.International archives of allergy and immunology · 2024Review
- Article
- Wildfire Smoke Exposure during Pregnancy: A Review of Potential Mechanisms of Placental Toxicity, Impact on Obstetric Outcomes, and Strategies to Reduce Exposure.International journal of environmental research and public health · 2022Review
- Indoor Air Quality Considerations for Laboratory Animals in Wildfire-Impacted Regions-A Pilot Study.Toxics · 2022Article
- Sustained Club Cell Injury in Mice Induces Histopathologic Features of Deployment-Related Constrictive Bronchiolitis.The American journal of pathology · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Our previous work has shown that coarse particulate matter (PM(10-2.5)) from wildfire smoke is more toxic to lung macrophages on an equal dose (by mass) basis than coarse PM isolated from normal ambient air, as evidenced by decreased numbers of macrophages in lung lavage fluid 6 and 24hours after PM instillation into mouse lungs in vivo and by cytotoxicity to a macrophage cell line observed directly in vitro. We hypothesized that pulmonary macrophages from mice instilled with wildfire coarse PM would undergo more cytotoxicity than macrophages from controls, and that there would be an increase in oxidative stress in their lungs. Cytotoxicity was quantified as decreased viable macrophages and increased percentages of dead macrophages in the bronchoalveolar lavage fluid (BALF) of mice instilled with wildfire coarse PM. At 1hour after PM instillation, we observed both decreased numbers of viable macrophages and increased dead macrophage percentages as compared to controls. An increase in free isoprostanes, an indicator of oxidative stress, from control values of 28.1±3.2pg/mL to 83.9±12.2pg/mL was observed a half-hour after PM instillation. By 1hour after PM instillation, isoprostane values had returned to 30.4±7.6pg/mL, not significantly different from control concentrations. Lung sections from mice instilled with wildfire coarse PM showed rapid Clara cell responses, with decreased intracellular staining for the Clara cell secretory protein CCSP 1hour after wildfire PM instillation. In conclusion, very rapid cytotoxicity occurs in pulmonary macrophages and oxidative stress responses are seen 0.5-1hour after wildfire coarse PM instillation. These results define early cellular and biochemical events occurring in vivo and support the hypothesis that oxidative stress-mediated macrophage toxicity plays a key role in the initial response of the mouse lung to wildfire PM exposure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.