ArticleCell2012
Protection from feed-forward amplification in an amplified RNAi mechanism.
Article in Cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed.
- Exogenous dsRNA made accessible to Dicer by two eukaryotic RNA-dependent RNA polymerases in Paramecium tetraurelia.Communications biology · 2026Article
- Characterization of RNA interference in the cnidarian Nematostella vectensis reveals partial target silencing but lack of small RNA amplification.PLoS biology · 2026Article
- Intraspecific variation in the duration of epigenetic inheritance.bioRxiv : the preprint server for biology · 2025Article
- Harnessing RNA interference for the control of Fusarium species: A critical review.Molecular plant pathology · 2024Review
- Article
- Target-specific requirements for RNA interference can arise through restricted RNA amplification despite the lack of specialized pathways.bioRxiv : the preprint server for biology · 2024Article
- Heritable epigenetic changes are constrained by the dynamics of regulatory architectures.eLife · 2024Article
- Nucleus-independent transgenerational small RNA inheritance inScience advances · 2023Article
- Article
- The conserved helicase ZNFX-1 memorializes silenced RNAs in perinuclear condensates.Nature cell biology · 2022Article
- Nuage condensates: accelerators or circuit breakers for sRNA silencing pathways?RNA (New York, N.Y.) · 2022Review
- shRNA transgenic swine display resistance to infection with the foot-and-mouth disease virus.Scientific reports · 2021Article
- Germ Granules Allow Transmission of Small RNA-Based Parental Responses in the "Germ Plasm".iScience · 2020Review
- Identification of oligo-adenylated small RNAs in the parasite Entamoeba and a potential role for small RNA control.BMC genomics · 2020Article
- Heritable Epigenetic Changes Alter Transgenerational Waveforms Maintained by Cycling Stores of Information.BioEssays : news and reviews in molecular, cellular and developmental biology · 2020Article
- Multigenerational Regulation of theAnnual review of genetics · 2019Review
- Transgenerational epigenetic inheritance: from phenomena to molecular mechanisms.Current opinion in neurobiology · 2019Review
- Gene silencing by double-stranded RNA from C. elegans neurons reveals functional mosaicism of RNA interference.Nucleic acids research · 2019Article
- Germ Granules Govern Small RNA Inheritance.Current biology : CB · 2019Article
- Host-induced silencing of Mi-msp-1 confers resistance to root-knot nematode Meloidogyne incognita in eggplant.Transgenic research · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The effectiveness of RNA interference (RNAi) in many organisms is potentiated through the signal-amplifying activity of a targeted RNA-directed RNA polymerase (RdRP) system that can convert a small population of exogenously-encountered dsRNA fragments into an abundant internal pool of small interfering RNA (siRNA). As for any biological amplification system, we expect an underlying architecture that will limit the ability of a randomly encountered trigger to produce an uncontrolled and self-escalating response. Investigating such limits in Caenorhabditis elegans, we find that feed-forward amplification is limited by biosynthetic and structural distinctions at the RNA level between (1) triggers that can produce amplification and (2) siRNA products of the amplification reaction. By assuring that initial (primary) siRNAs can act as triggers but not templates for activation, and that the resulting (secondary) siRNAs can enforce gene silencing on additional targets without unbridled trigger amplification, the system achieves substantial but fundamentally limited signal amplification.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.