ArticlePlant biotechnology journal2013
Engineering secondary cell wall deposition in plants.
Article in Plant biotechnology journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed.
- Pan-genome characterization of the maize 4CL gene family and its dynamic responses to abiotic stress.Plant molecular biology · 2026Article
- Multi-omics reveals growth regulatory mechanisms induced by exogenous TgSWO expansin-like protein in strawberry.Frontiers in plant science · 2026Article
- Transcriptome and targeted metabolome analysis reveals sugar metabolism regulation in maize stalks at different densities.Frontiers in plant science · 2026Article
- Comprehensive analysis of the NAC transcription factor gene family in Sophora tonkinensis Gagnep.BMC plant biology · 2025Article
- Synthetic-biology approach for plant lignocellulose engineering.Plant biotechnology (Tokyo, Japan) · 2024Article
- Genome-Wide Identification and Functional Analysis of the GUX Gene Family inInternational journal of molecular sciences · 2024Article
- Modifying lignin composition and xylan O-acetylation induces changes in cell wall composition, extractability, and digestibility.Biotechnology for biofuels and bioproducts · 2024Article
- The Carbon Flow Shifts from Primary to Secondary Metabolism during Xylem Vessel Cell Differentiation in Arabidopsis thaliana.Plant & cell physiology · 2023Article
- Comprehensive Time-Course Transcriptome Reveals the Crucial Biological Pathways Involved in the Seasonal Branch Growth in Siberian Elm (International journal of molecular sciences · 2023Article
- Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar.Nature communications · 2023Article
- Microscopic and metabolic investigations disclose the factors that lead to skin cracking in chili-type pepper fruit varieties.Horticulture research · 2023Article
- Lignin engineering in forest trees: From gene discovery to field trials.Plant communications · 2022Review
- Aspartic proteases modulate programmed cell death and secondary cell wall synthesis during wood formation in poplar.Journal of experimental botany · 2022Article
- Overexpression ofInternational journal of molecular sciences · 2022Article
- Spatio-Temporal Modification of Lignin Biosynthesis in Plants: A Promising Strategy for Lignocellulose Improvement and Lignin Valorization.Frontiers in bioengineering and biotechnology · 2022Review
- Enhancement of Secondary Cell Wall Formation in Poplar Xylem Using a Self-Reinforced System of Secondary Cell Wall-Related Transcription Factors.Frontiers in plant science · 2022Article
- CRISPR-Cas9 editing of CAFFEOYL SHIKIMATE ESTERASE 1 and 2 shows their importance and partial redundancy in lignification in Populus tremula × P. alba.Plant biotechnology journal · 2021Article
- Field evaluation of transgenic hybrid poplars with desirable wood properties and enhanced growth for biofuel production by bicistronic expression of PdGA20ox1 and PtrMYB3 in wood-forming tissue.Biotechnology for biofuels · 2021Article
- Tailoring renewable materials via plant biotechnology.Biotechnology for biofuels · 2021Review
- The thickening and modification of the galactan-enriched layer during primary phloem fibre development inAoB PLANTS · 2021Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lignocellulosic biomass was used for thousands of years as animal feed and is now considered a great sugar source for biofuels production. It is composed mostly of secondary cell walls built with polysaccharide polymers that are embedded in lignin to reinforce the cell wall structure and maintain its integrity. Lignin is the primary material responsible for biomass recalcitrance to enzymatic hydrolysis. During plant development, deep reductions of lignin cause growth defects and often correlate with the loss of vessel integrity that adversely affects water and nutrient transport in plants. The work presented here describes a new approach to decrease lignin content while preventing vessel collapse and introduces a new strategy to boost transcription factor expression in native tissues. We used synthetic biology tools in Arabidopsis to rewire the secondary cell network by changing promoter-coding sequence associations. The result was a reduction in lignin and an increase in polysaccharide depositions in fibre cells. The promoter of a key lignin gene, C4H, was replaced by the vessel-specific promoter of transcription factor VND6. This rewired lignin biosynthesis specifically for vessel formation while disconnecting C4H expression from the fibre regulatory network. Secondly, the promoter of the IRX8 gene, secondary cell wall glycosyltransferase, was used to express a new copy of the fibre transcription factor NST1, and as the IRX8 promoter is induced by NST1, this also created an artificial positive feedback loop (APFL). The combination of strategies-lignin rewiring with APFL insertion-enhances polysaccharide deposition in stems without over-lignifying them, resulting in higher sugar yields after enzymatic hydrolysis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.