ArticleAnalytical chemistry2012
A droplet-based, optofluidic device for high-throughput, quantitative bioanalysis.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- A Simple Pump-Free Approach to Generating High-Throughput Microdroplets Using Oscillating Microcone Arrays.Micromachines · 2024Article
- On-Chip Photonic Detection Techniques for Non-Invasive In Situ Characterizations at the Microfluidic Scale.Sensors (Basel, Switzerland) · 2024Review
- Multiplexed fluorescence and scatter detection with single cell resolution using on-chip fiber optics for droplet microfluidic applications.Microsystems & nanoengineering · 2024Article
- Acoustofluidic assembly of primary tumor-derived organotypic cell clusters for rapid evaluation of cancer immunotherapy.Journal of nanobiotechnology · 2023Article
- Droplet Detection and Sorting System in Microfluidics: A Review.Micromachines · 2022Review
- Microfluidic-based processors and circuits design.Scientific reports · 2021Article
- Optofluidic detection setup for multi-parametric analysis of microbiological samples in droplets.Biomicrofluidics · 2020Article
- From single-molecule detection to next-generation sequencing: microfluidic droplets for high-throughput nucleic acid analysis.Microfluidics and nanofluidics · 2017Article
- Switchable 3D optofluidic Y-branch waveguides tuned by Dean flows.Scientific reports · 2016Article
- Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers.Journal of visualized experiments : JoVE · 2016Article
- Compact and modular multicolour fluorescence detector for droplet microfluidics.Lab on a chip · 2015Article
- Image decoding of photonic crystal beads array in the microfluidic chip for multiplex assays.Scientific reports · 2014Article
- DNA hybridization detection with 100 zM sensitivity using piezoelectric plate sensors with an improved noise-reduction algorithm.The Analyst · 2014Article
- An acoustofluidic micromixer via bubble inception and cavitation from microchannel sidewalls.Analytical chemistry · 2014Article
- Article
- Standing surface acoustic wave (SSAW)-based microfluidic cytometer.Lab on a chip · 2014Article
- An acoustofluidic micromixer based on oscillating sidewall sharp-edges.Lab on a chip · 2013Article
- Lab-on-a-chip technologies for single-molecule studies.Lab on a chip · 2013Article
- An on-chip, multichannel droplet sorter using standing surface acoustic waves.Analytical chemistry · 2013Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Analysis of chemical or biomolecular contents in a tiny amount of specimen presents a significant challenge in many biochemical studies and diagnostic applications. In this work, we present a single-layer, optofluidic device for real-time, high-throughput, quantitative analysis of droplet contents. Our device integrates an optical fiber-based, on-chip detection unit with a droplet-based microfluidic unit. It can quantitatively analyze the contents of individual droplets in real-time. It also achieves a detection throughput of 2000 droplets per second, a detection limit of 20 nM, and an excellent reproducibility in its detection results. In a proof-of-concept study, we demonstrate that our device can be used to perform detection of DNA and its mutations by monitoring the fluorescent signal changes of the target DNA/molecular beacon complex in single droplets. Our approach can be immediately extended to a real-time, high-throughput detection of other biomolecules (such as proteins and viruses) in droplets. With its advantages in throughput, functionality, cost, size, and reliability, the droplet-based optofluidic device presented here can be a valuable tool for many medical diagnostic applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.