ArticleJournal of microscopy2012
Three-dimensional reconstructions from optical sections of thick mouse inner ears using confocal microscopy.
Article in Journal of microscopy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Obtaining 3D super-resolution images by utilizing rotationally symmetric structures and 2D-to-3D transformation.Computational and structural biotechnology journal · 2023Review
- Article
- Evaluation of the Efficacy of Dexamethasone-Eluting Electrode Array on the Post-Implant Cochlear Fibrotic Reaction by Three-Dimensional Immunofluorescence Analysis in Mongolian Gerbil Cochlea.Journal of clinical medicine · 2021Article
- In Situ 3D-Imaging of the Inner Ear Synapses with a Cochlear Implant.Life (Basel, Switzerland) · 2021Article
- Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1Stem cell research & therapy · 2021Article
- Effects of Neurod1 Expression on Mouse and Human Schwannoma Cells.The Laryngoscope · 2021Article
- Interaction with ectopic cochlear crista sensory epithelium disrupts basal cochlear sensory epithelium development in Lmx1a mutant mice.Cell and tissue research · 2020Article
- Imaging Bioluminescent Exogenous Stem Cells in the Intact Guinea Pig Cochlea.Anatomical record (Hoboken, N.J. : 2007) · 2020Article
- Early ear neuronal development, but not olfactory or lens development, can proceed without SOX2.Developmental biology · 2020Article
- Topologically correct central projections of tetrapod inner ear afferents require Fzd3.Scientific reports · 2019Article
- Transplantation of Ears Provides Insights into Inner Ear Afferent Pathfinding Properties.Developmental neurobiology · 2018Article
- Ear transplantations reveal conservation of inner ear afferent pathfinding cues.Scientific reports · 2018Article
- Sonic hedgehog antagonists reduce size and alter patterning of the frog inner ear.Developmental neurobiology · 2017Article
- Incomplete and delayed Sox2 deletion defines residual ear neurosensory development and maintenance.Scientific reports · 2016Article
- Three/four-dimensional (3D/4D) microscopic imaging and processing in clinical dental research.BMC oral health · 2016Article
- Neuroanatomical Tracing Techniques in the Ear: History, State of the Art, and Future Developments.Methods in molecular biology (Clifton, N.J.) · 2016Review
- Ear manipulations reveal a critical period for survival and dendritic development at the single-cell level in Mauthner neurons.Developmental neurobiology · 2015Article
- Comparison of traditional histology and TSLIM optical sectioning of human temporal bones.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2014Article
- HMGA2, the architectural transcription factor high mobility group, is expressed in the developing and mature mouse cochlea.PloS one · 2014Article
- Postnatal refinement of auditory hair cell planar polarity deficits occurs in the absence of Vangl2.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Three-dimensional (3D) reconstructions of the vertebrate inner ear have provided novel insights into the development of this complex organ. 3D reconstructions enable superior analysis of phenotypic differences between wild type and mutant ears but can result in laborious work when reconstructed from physically sectioned material. Although nondestructive optical sectioning light sheet microscopy may ultimately prove the ideal solution, these technologies are not yet commercially available, or in many instances are not monetarily feasible. Here we introduce a simple technique to image a fluorescently labelled ear at different stages throughout development at high resolution enabling 3D reconstruction of any component of the inner ear using confocal microscopy. We provide a step-by-step manual from tissue preparation to imaging to 3D reconstruction and analysis including a rationale and troubleshooting guide at each step for researchers with different equipment, protocols, and access to resources to successfully incorporate the principles of this method and customize them to their laboratory settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.