ArticleScientific reports2012
A functioning artificial secretory cell.
Article in Scientific reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Electrochemical Droplet Sculpturing of Short Carbon Fiber Nanotip Electrodes for Neurotransmitter Detection.ACS electrochemistry · 2025Article
- Digging into the biophysical features of cell membranes with lipid-DNA conjugates.Quarterly reviews of biophysics · 2022Review
- Recent Advances in Liposome-Based Molecular Robots.Micromachines · 2020Review
- Amperometry methods for monitoring vesicular quantal size and regulation of exocytosis release.Pflugers Archiv : European journal of physiology · 2018Review
- Interpretation of amperometric kinetics of content release during contacts of vesicles with a lipid membrane.European biophysics journal : EBJ · 2017Article
- DNA Nanostructures on Membranes as Tools for Synthetic Biology.Biophysical journal · 2016Review
- All-carbon multi-electrode array for real-time in vitro measurements of oxidizable neurotransmitters.Scientific reports · 2016Article
- Analysis of interactions between SNARE proteins using imaging ellipsometer coupled with microfluidic array.Scientific reports · 2014Article
- Actin controls the vesicular fraction of dopamine released during extended kiss and run exocytosis.ACS chemical biology · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present an amperometric study of content release from individual vesicles in an artificial secretory cell designed with the minimal components required to carry out exocytosis. Here, the membranes of the cell and vesicles are substituted for protein-free giant and large unilamellar vesicles respectively. In replacement of the SNARE-complex, the cell model was equipped with an analog composed of complimentary DNA constructs. The DNA constructs hybridize in a zipper-like fashion to bring about docking of the artificial secretory vesicles and following the addition of Ca(2+ )artificial exocytosis was completed. Exocytotic events recorded from the artificial cell closely approximate exocytosis in live cells. The results together with simulations of vesicular release demonstrate that the molecular flux in this model is attenuated and we suggest that this is the result of restricted diffusion through a semi-stable fusion pore or a partitioning of the signalling molecule out of the fused vesicle membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.