ArticlePloS one2012
Functional characterization of calcineurin homologs PsCNA1/PsCNB1 in Puccinia striiformis f. sp. tritici using a host-induced RNAi system.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 47 citations in OpenAlex.
- Transcriptome Analysis and Functional Characterization of thePlants (Basel, Switzerland) · 2025Article
- RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi.International journal of molecular sciences · 2023Review
- Host induced gene silencing ofFrontiers in plant science · 2022Article
- Recent advances and potential applications of cross-kingdom movement of miRNAs in modulating plant's disease response.RNA biology · 2022Review
- Threat at One End of the Plant: What Travels to Inform the Other Parts?International journal of molecular sciences · 2021Review
- Movement of small RNAs in and between plants and fungi.Molecular plant pathology · 2020Review
- Calcineurin Regulates Conidiation, Chlamydospore Formation and Virulence inFrontiers in microbiology · 2020Article
- Calcium-Calmodulin-Calcineurin Signaling: A Globally Conserved Virulence Cascade in Eukaryotic Microbial Pathogens.Cell host & microbe · 2019Review
- Review
- Host-Induced Gene Silencing ofFrontiers in plant science · 2019Article
- Cross-kingdom RNA trafficking and environmental RNAi-nature's blueprint for modern crop protection strategies.Current opinion in microbiology · 2018Review
- Host-induced silencing of essential genes in Puccinia triticina through transgenic expression of RNAi sequences reduces severity of leaf rust infection in wheat.Plant biotechnology journal · 2018Article
- Host-induced gene silencing compromises Verticillium wilt in tomato and Arabidopsis.Molecular plant pathology · 2018Article
- Host-induced silencing of Fusarium culmorum genes protects wheat from infection.Journal of experimental botany · 2016Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
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Abstract
Calcineurin plays a key role in morphogenesis, pathogenesis and drug resistance in most fungi. However, the function of calcineurin genes in Puccinia striiformis f. sp. tritici (Pst) is unclear. We identified and characterized the calcineurin genes PsCNA1 and PsCNB1 in Pst. Phylogenetic analyses indicate that PsCNA1 and PsCNB1 form a calcium/calmodulin regulated protein phosphatase belonging to the calcineurin heterodimers composed of subunits A and B. Quantitative RT-PCR analyses revealed that both PsCNA1 and PsCNB1 expression reached their maximum in the stage of haustorium formation, which is one day after inoculation. Using barely stripe mosaic virus (BSMV) as a transient expression vector in wheat, the expression of PsCNA1 and PsCNB1 in Pst was suppressed, leading to slower extension of fungal hyphae and reduced production of urediospores. The immune-suppressive drugs cyclosporin A and FK506 markedly reduced the germination rates of urediospores, and when germination did occur, more than two germtubes were produced. These results suggest that the calcineurin signaling pathway participates in stripe rust morphogenetic differentiation, especially the formation of haustoria during the early stage of infection and during the production of urediospores. Therefore PsCNA1 and PsCNB1 can be considered important pathogenicity genes involved in the wheat-Pst interaction.
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