ArticlePloS one2012
Library preparation and multiplex capture for massive parallel sequencing applications made efficient and easy.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Potential Adaptive Introgression From Dogs in Iberian Grey Wolves (Canis lupus).Molecular ecology · 2025Article
- Reducing Supply Chain Dependencies for Viral Genomic Surveillance: Get by with a Little HELP from Commercial Enzymes already in your Lab Freezer.Wellcome open research · 2025Article
- Large variance in inbreeding within the Iberian wolf population.The Journal of heredity · 2024Article
- Chromosome evolution screens recapitulate tissue-specific tumor aneuploidy patterns.Nature genetics · 2024Article
- Article
- High-Throughput Variant Detection Using a Color-Mixing Strategy.The Journal of molecular diagnostics : JMD · 2022Article
- Construction of Strand-seq libraries in open nanoliter arrays.Cell reports methods · 2022Article
- Pre-capture multiplexing provides additional power to detect copy number variation in exome sequencing.BMC bioinformatics · 2021Article
- Off-Target Analysis in Gene Editing and Applications for Clinical Translation of CRISPR/Cas9 in HIV-1 Therapy.Frontiers in genome editing · 2021Review
- Fluorescent amplification for next generation sequencing (FA-NGS) library preparation.BMC genomics · 2020Article
- Cost-effective detection of genome-wide signatures for 2,4-D herbicide resistance adaptation in red clover.Scientific reports · 2019Article
- Automated in situ chromatin profiling efficiently resolves cell types and gene regulatory programs.Epigenetics & chromatin · 2018Article
- Understanding the Complexity of Cold Tolerance in White Clover using Temperature Gradient Locations and a GWAS Approach.The plant genome · 2018Article
- Simple and Complex Centromeric Satellites inGenetics · 2018Article
- A comprehensive custom panel design for routine hereditary cancer testing: preserving control, improving diagnostics and revealing a complex variation landscape.Scientific reports · 2017Article
- SELMAP - SELEX affinity landscape MAPping of transcription factor binding sites using integrated microfluidics.Scientific reports · 2016Article
- Nuclear genomic signals of the 'microturbellarian' roots of platyhelminth evolutionary innovation.eLife · 2015Article
- Whole genome prediction for preimplantation genetic diagnosis.Genome medicine · 2015Article
- Anchored multiplex PCR for targeted next-generation sequencing.Nature medicine · 2014Article
- Validation and implementation of targeted capture and sequencing for the detection of actionable mutation, copy number variation, and gene rearrangement in clinical cancer specimens.The Journal of molecular diagnostics : JMD · 2014Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the recent years, rapid development of sequencing technologies and a competitive market has enabled researchers to perform massive sequencing projects at a reasonable cost. As the price for the actual sequencing reactions drops, enabling more samples to be sequenced, the relative price for preparing libraries gets larger and the practical laboratory work becomes complex and tedious. We present a cost-effective strategy for simplified library preparation compatible with both whole genome- and targeted sequencing experiments. An optimized enzyme composition and reaction buffer reduces the number of required clean-up steps and allows for usage of bulk enzymes which makes the whole process cheap, efficient and simple. We also present a two-tagging strategy, which allows for multiplex sequencing of targeted regions. To prove our concept, we have prepared libraries for low-pass sequencing from 100 ng DNA, performed 2-, 4- and 8-plex exome capture and a 96-plex capture of a 500 kb region. In all samples we see a high concordance (>99.4%) of SNP calls when comparing to commercially available SNP-chip platforms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.