ArticlePloS one2012
The human homolog of Escherichia coli endonuclease V is a nucleolar protein with affinity for branched DNA structures.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- ENDONUCLEASE V: FROM TRANSCRIPTOME REGULATOR TO CHEMICAL BIOLOGY TOOL.ChemistryEurope · 2026Article
- Biochemical and mutational studies of an endonuclease V from the hyperthermophilic crenarchaeon Sulfolobus islandicus REY15A.World journal of microbiology & biotechnology · 2023Article
- Review
- Deletion of Endonuclease V suppresses chemically induced hepatocellular carcinoma.Nucleic acids research · 2020Article
- Selective Enrichment of A-to-I Edited Transcripts from Cellular RNA Using Endonuclease V.Journal of the American Chemical Society · 2020Article
- Complex alternative splicing of human Endonuclease V mRNA, but evidence for only a single protein isoform.PloS one · 2019Article
- Breaking the speed limit with multimode fast scanning of DNA by Endonuclease V.Nature communications · 2018Article
- Insights into the role of endonuclease V in RNA metabolism in Trypanosoma brucei.Scientific reports · 2017Article
- Regulation of Human Endonuclease V Activity and Relocalization to Cytoplasmic Stress Granules.The Journal of biological chemistry · 2016Article
- Deoxyinosine triphosphate induces MLH1/PMS2- and p53-dependent cell growth arrest and DNA instability in mammalian cells.Scientific reports · 2016Article
- Crystal structure and MD simulation of mouse EndoV reveal wedge motif plasticity in this inosine-specific endonuclease.Scientific reports · 2016Article
- Crystal structure of E. coli endonuclease V, an essential enzyme for deamination repair.Scientific reports · 2015Article
- The cutting edges in DNA repair, licensing, and fidelity: DNA and RNA repair nucleases sculpt DNA to measure twice, cut once.DNA repair · 2014Review
- Endonuclease V cleaves at inosines in RNA.Nature communications · 2013Article
- Human endonuclease V is a ribonuclease specific for inosine-containing RNA.Nature communications · 2013Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Loss of amino groups from adenines in DNA results in the formation of hypoxanthine (Hx) bases with miscoding properties. The primary enzyme in Escherichia coli for DNA repair initiation at deaminated adenine is endonuclease V (endoV), encoded by the nfi gene, which cleaves the second phosphodiester bond 3' of an Hx lesion. Endonuclease V orthologs are widespread in nature and belong to a family of highly conserved proteins. Whereas prokaryotic endoV enzymes are well characterized, the function of the eukaryotic homologs remains obscure. Here we describe the human endoV ortholog and show with bioinformatics and experimental analysis that a large number of transcript variants exist for the human endonuclease V gene (ENDOV), many of which are unlikely to be translated into functional protein. Full-length ENDOV is encoded by 8 evolutionary conserved exons covering the core region of the enzyme, in addition to one or more 3'-exons encoding an unstructured and poorly conserved C-terminus. In contrast to the E. coli enzyme, we find recombinant ENDOV neither to incise nor bind Hx-containing DNA. While both enzymes have strong affinity for several branched DNA substrates, cleavage is observed only with E. coli endoV. We find that ENDOV is localized in the cytoplasm and nucleoli of human cells. As nucleoli harbor the rRNA genes, this may suggest a role for the protein in rRNA gene transactions such as DNA replication or RNA transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.