ArticleInternational journal of biological sciences2012
Characterization of a chitin synthase encoding gene and effect of diflubenzuron in soybean aphid, Aphis glycines.
Article in International journal of biological sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 68 citations in OpenAlex.
- Two-way transcriptome approach for the identification of common gene targets across four insect orders and its validation in Oxycarenus laetus.Scientific reports · 2025Article
- Host-specific co-evolution likely driven by diet in Buchnera aphidicola.BMC genomics · 2024Article
- Characterization of Chitin Synthase A cDNA fromInsects · 2022Article
- Molecular Characterization of Chitin Synthase Gene inInsects · 2021Article
- Chitin Synthases Are Critical for Reproduction, Molting, and Digestion in the Salmon Louse (Life (Basel, Switzerland) · 2021Article
- A patent review on strategies for biological control of mosquito vector.World journal of microbiology & biotechnology · 2020Review
- Identification, Characterization, and Functional Analysis of Chitin Synthase Genes inInternational journal of molecular sciences · 2020Article
- Identification and Functional Study of Chitin Metabolism and Detoxification-Related Genes inInternational journal of molecular sciences · 2020Article
- Article
- Insect Cuticular Chitin Contributes to Form and Function.Current pharmaceutical design · 2020Article
- RNAi-Mediated Knockdown of Chitin Synthase 1 (Insects · 2019Article
- Impact of low lethal concentrations of buprofezin on biological traits and expression profile of chitin synthase 1 gene (CHS1) in melon aphid, Aphis gossypii.Scientific reports · 2019Article
- Silencing of the Chitin Synthase Gene Is Lethal to the Asian Citrus Psyllid,International journal of molecular sciences · 2019Article
- Effects of RNAi-based silencing of chitin synthase gene on moulting and fecundity in pea aphids (Acyrthosiphon pisum).Scientific reports · 2019Article
- Molecular cloning, expression, and functional analysis of the chitin synthase 1 gene and its two alternative splicing variants in the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae).Scientific reports · 2019Article
- Identification and validation of reference genes for gene expression analysis in Aphidius gifuensis (Hymenoptera: Aphidiidae).PloS one · 2017Article
- Comprehensive evaluation of candidate reference genes for qRT-PCR studies of gene expression in mustard aphid, Lipaphis erysimi (Kalt).Scientific reports · 2016Article
- Exposure to diflubenzuron results in an up-regulation of a chitin synthase 1 gene in citrus red mite, Panonychus citri (Acari: Tetranychidae).International journal of molecular sciences · 2014Article
- Article
- Core RNAi Machinery and Sid1, a Component for Systemic RNAi, in the Hemipteran Insect, Aphis glycines.International journal of molecular sciences · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitin synthases are critical enzymes for synthesis of chitin and thus for subsequent growth and development in insects. We identified the cDNA of chitin synthase gene (CHS) in Aphis glycines, the soybean aphid, which is a serious pest of soybean. The full-length cDNA of CHS in A. glycines (AyCHS) was 5802 bp long with an open reading frame of 4704 bp that encoded for a 1567 amino acid residues protein. The predicted AyCHS protein had a molecular mass of 180.05 kDa and its amino acid sequence contained all the signature motifs (EDR, QRRRW and TWGTR) of chitin synthases. The quantitative real-time PCR (qPCR) analysis revealed that AyCHS was expressed in all major tissues (gut, fat body and integument); however, it had the highest expression in integument (~3.5 fold compared to gut). Interestingly, the expression of AyCHS in developing embryos was nearly 7 fold higher compared to adult integument, which probably is a reflection of embryonic molts in hemimetabolus insects. Expression analysis in different developmental stages of A. glycines revealed a consistent AyCHS expression in all stages. Further, through leaf dip bioassay, we tested the effect of diflubenzuron (DFB, Dimilin ®), a chitin-synthesis inhibitor, on A. glycines' survival, fecundity and body weight. When fed with soybean leaves previously dipped in 50 ppm DFB solution, A. glycines nymphs suffered significantly higher mortality compared to control. A. glycines nymphs feeding on diflubenzuron treated leaves showed a slightly enhanced expression (1.67 fold) of AyCHS compared to nymphs on untreated leaves. We discussed the potential applications of the current study to develop novel management strategies using chitin-synthesis inhibitors and using RNAi by knocking down AyCHS expression.
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