ArticleMicrobiology (Reading, England)2013
A supplemented soft agar chemotaxis assay demonstrates the Helicobacter pylori chemotactic response to zinc and nickel.
Article in Microbiology (Reading, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Ergosterol from Yeasts PromotesInternational journal of molecular sciences · 2026Article
- The E. coli escape wave in response to external ZnBiometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2025Article
- Low pH amplifies chemotaxis toward urea in Helicobacter pylori.Biophysical journal · 2025Article
- Genetic Basis of Gap Formation Between MigratingMicroorganisms · 2025Article
- Comparative Genomic Insights into the Evolution ofmSystems · 2022Article
- Insights into the Orchestration of Gene Transcription Regulators inInternational journal of molecular sciences · 2022Review
- Multiple detection of both attractants and repellents by the dCache-chemoreceptor SO_1056 of Shewanella oneidensis.The FEBS journal · 2022Article
- A Bacterial Inflammation Sensor Regulates c-di-GMP Signaling, Adhesion, and Biofilm Formation.mBio · 2021Article
- A multiscale 3D chemotaxis assay reveals bacterial navigation mechanisms.Communications biology · 2021Article
- Asymmetric random walks reveal that the chemotaxis network modulates flagellar rotational bias ineLife · 2021Article
- Metal homeostasis in pathogenic Epsilonproteobacteria: mechanisms of acquisition, efflux, and regulation.Metallomics : integrated biometal science · 2021Review
- Long-chain fatty acids alter transcription ofPeerJ · 2021Article
- The role of solute binding proteins in signal transduction.Computational and structural biotechnology journal · 2021Review
- On-chip MIC by Combining Concentration Gradient Generator and Flanged Chamber Arrays.Micromachines · 2020Article
- Colonization, localization, and inflammation: the roles of H. pylori chemotaxis in vivo.Current opinion in microbiology · 2018Review
- Transducer-Like Protein inFrontiers in microbiology · 2018Article
- Sensory Repertoire of Bacterial Chemoreceptors.Microbiology and molecular biology reviews : MMBR · 2017Review
- Helicobacter pylori chemoreceptor TlpC mediates chemotaxis to lactate.Scientific reports · 2017Article
- Campylobacter jejuni transducer like proteins: Chemotaxis and beyond.Gut microbes · 2017Review
- Multiple Acid Sensors Control Helicobacter pylori Colonization of the Stomach.PLoS pathogens · 2017Article
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3 authors.
Funding
Abstract
Directed motility, or chemotaxis, is required for Helicobacter pylori to establish infection in the stomach, although the full repertoire of this bacterium's chemotactic responses is not yet known. Here we report that H. pylori responds to zinc as an attractant and nickel as a repellent. To reach this conclusion, we employed both a temporal chemotaxis assay based on bacterial reversals and a supplemented soft agar spatial assay. We refined the temporal assay using a previously described chemorepellent, acid, and found that H. pylori requires rich media with serum to maintain optimal swimming motility. Surprisingly, we found that some strains respond to acid as an attractant, and that the TlpC chemoreceptor correlated with whether acid was sensed as an attractant or repellent. Using this same assay, we detected weak repellent responses to nickel and copper, and a varied response to zinc. We thus developed an alternative spatial chemotactic assay called the supplemented soft agar assay, which utilizes soft agar medium supplemented with the test compound. With Escherichia coli, the attractant serine slowed overall bacterial migration, while the repellent nickel increased the speed of overall migration. In H. pylori we detected slowed migration with doubled tryptone media, as well as zinc, consistent with an attractant response. In contrast, nickel increased migration, consistent with repulsion.
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