ArticleScience (New York, N.Y.)2012
Optical control of protein activity by fluorescent protein domains.
Article in Science (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 156 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
156 citing papers in PubMed, 290 citations in OpenAlex.
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- Recent Advances in the ESIPT-based Responsive Probes for the Detection of Bio-Active Molecules CO, NO and H₂S.Journal of fluorescence · 2026Review
- Optogenetic control of transition to metamorphosis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Interrogating Physiological Functions with Light and Chemicals.Annual review of physiology · 2026Review
- Red-Light-Only Control of Protein-Protein Interactions Using a Cyanobacteriochrome (UNICYCL).ACS central science · 2026Article
- Dynamics and Mechanism of Off- to On-Switching in Dreiklang a Decoupled Reversibly Switchable Fluorescent Protein.Angewandte Chemie (International ed. in English) · 2026Article
- The p.L218P variant in WDR83 disrupts neuronal development, leading to neurodevelopmental disorder.Scientific reports · 2025Article
- Environment signal dependent biocontainment systems for engineered organisms: Leveraging triggered responses and combinatorial systems.Synthetic and systems biotechnology · 2025Review
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- Optical Control of Microtubule Accumulation and Dispersion by Tau-Derived Peptide-Fused Photoresponsive Protein.JACS Au · 2025Article
- Reversible light-responsive protein hydrogel for on-demand cell encapsulation and release.Acta biomaterialia · 2025Article
- Autonomous Nucleic Acid and Protein Nanocomputing Agents Engineered to Operate in Living Cells.ACS nano · 2025Review
- Chemical control of excited-state reactivity of the anionic green fluorescent protein chromophore.Communications chemistry · 2024Article
- Recent Updates of the CRISPR/Cas9 Genome Editing System: Novel Approaches to Regulate Its Spatiotemporal Control by Genetic and Physicochemical Strategies.International journal of nanomedicine · 2024Review
- Stimuli-Responsive Protein Hydrogels: Their Design, Properties, and Biomedical Applications.Polymers · 2023Review
- Article
- Optical control of ultrafast structural dynamics in a fluorescent protein.Nature chemistry · 2023Article
- Allosteric regulation of kinase activity in living cells.bioRxiv : the preprint server for biology · 2023Article
- Quantitative insights in tissue growth and morphogenesis with optogenetics.Physical biology · 2023Review
- Kinase-Modulated Bioluminescent Indicators Enable Noninvasive Imaging of Drug Activity in the Brain.ACS central science · 2023Article
96 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescent proteins (FPs) are widely used as optical sensors, whereas other light-absorbing domains have been used for optical control of protein localization or activity. Here, we describe light-dependent dissociation and association in a mutant of the photochromic FP Dronpa, and we used it to control protein activities with light. We created a fluorescent light-inducible protein design in which Dronpa domains are fused to both termini of an enzyme domain. In the dark, the Dronpa domains associate and cage the protein, but light induces Dronpa dissociation and activates the protein. This method enabled optical control over guanine nucleotide exchange factor and protease domains without extensive screening. Our findings extend the applications of FPs from exclusively sensing functions to also encompass optogenetic control.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.