ArticleNature protocols2012
Live imaging of cellular dynamics during Caenorhabditis elegans postembryonic development.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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Who cites it
48 citing papers in PubMed, 83 citations in OpenAlex.
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- FGF diffusion is required for directed migration of postembryonic muscle progenitors in C. elegans.Development (Cambridge, England) · 2025Article
- Phosphorylation-dependent regional motility of the ciliary kinesin OSM-3.The Journal of cell biology · 2025Article
- Parameters that influence bipartite reporter system expression in Caenorhabditis elegans.Genetics · 2025Article
- MONITTR allows real-time imaging of transcription and endogenous proteins in C. elegans.The Journal of cell biology · 2025Article
- Visualization and Analyses of Cytoplasmic Streaming in C. elegans Zygotes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Endocytosis restricts dendrite branching via removing ectopically localized branching ligands.Nature communications · 2024Article
- Vacuolar HeLife · 2024Article
- Inhibiting HSD17B8 suppresses the cell proliferation caused by PTEN failure.Scientific reports · 2024Article
- Video-rate mid-infrared photothermal imaging by single-pulse photothermal detection per pixel.Science advances · 2023Article
- The cell cortex-localized protein CHDP-1 is required for dendritic development and transport in C. elegans neurons.PLoS genetics · 2022Article
- Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy.Genetics · 2022Review
- Live imaging of postembryonic developmental processes inSTAR protocols · 2022Article
- Dual-expression system for blue fluorescent protein optimization.Scientific reports · 2022Article
- In situ structure of intestinal apical surface reveals nanobristles on microvilli.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Wnt signaling polarizes cortical actin polymerization to increase daughter cell asymmetry.Cell discovery · 2022Article
- Live-cell Imaging and Analysis of Germline Stem Cell Mitosis inBio-protocol · 2022Article
- Bioactive Phytochemicals with Anti-Aging and Lifespan Extending Potentials inMolecules (Basel, Switzerland) · 2021Review
- Mechanistic insights into central spindle assembly mediated by the centralspindlin complex.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Actin filament debranching regulates cell polarity during cell migration and asymmetric cell division.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Postembryonic development is an important process of organismal maturation after embryonic growth. Despite key progress in recent years in understanding embryonic development via fluorescence time-lapse microscopy, comparatively less live-cell imaging of postembryonic development has been done. Here we describe a protocol to image larval development in the nematode Caenorhabditis elegans. Our protocol describes the construction of fluorescent transgenic C. elegans, immobilization of worm larvae and time-lapse microscopy analysis. To improve the throughput of imaging, we developed a C. elegans triple-fluorescence imaging approach with a worm-optimized blue fluorescent protein (TagBFP), green fluorescent protein (GFP) and mCherry. This protocol has been previously applied to time-lapse imaging analysis of Q neuroblast asymmetric division, migration and apoptosis, and we show here that it can also be used to image neuritogenesis in the L1 larvae. Other applications are also possible. The protocol can be completed within 3 h and may provide insights into understanding postembryonic development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.