ReviewPflugers Archiv : European journal of physiology2013
Multiplexed visualization of dynamic signaling networks using genetically encoded fluorescent protein-based biosensors.
Review in Pflugers Archiv : European journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding.iScience · 2025Article
- Genetically encoded fluorescent sensors for imaging neuronal dynamics in vivo.Journal of neurochemistry · 2023Review
- Seeing Neurodegeneration in a New Light Using Genetically Encoded Fluorescent Biosensors and iPSCs.International journal of molecular sciences · 2023Review
- Article
- Article
- Simultaneous readout of multiple FRET pairs using photochromism.Nature communications · 2021Article
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities.Nature cell biology · 2018Article
- Article
- Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Quantitative imaging of Rac1 activity in Dictyostelium cells with a fluorescently labelled GTPase-binding domain from DPAKa kinase.Histochemistry and cell biology · 2016Article
- A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo.Nature biotechnology · 2016Article
- Imaging GFP-based reporters in neurons with multiwavelength optogenetic control.Biophysical journal · 2014Article
- Genetically encoded fluorescent biosensors for live-cell visualization of protein phosphorylation.Chemistry & biology · 2014Review
- Monitoring of post-translational modification dynamics with genetically encoded fluorescent reporters.Biopolymers · 2014Review
- Retrograde labeling, transduction, and genetic targeting allow cellular analysis of corticospinal motor neurons: implications in health and disease.Frontiers in neuroanatomy · 2014Review
- Simultaneous mapping of membrane voltage and calcium in zebrafish heart in vivo reveals chamber-specific developmental transitions in ionic currents.Frontiers in physiology · 2014Article
- A multiplexed fluorescent assay for independent second-messenger systems: decoding GPCR activation in living cells.Journal of biomolecular screening · 2013Article
- Transgenic mouse lines for non-invasive ratiometric monitoring of intracellular chloride.Frontiers in molecular neuroscience · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Cells rely on a complex, interconnected network of signaling pathways to sense and interpret changes in their extracellular environment. The development of genetically encoded fluorescent protein (FP)-based biosensors has made it possible for researchers to directly observe and characterize the spatiotemporal dynamics of these intracellular signaling pathways in living cells. However, detailed information regarding the precise temporal and spatial relationships between intersecting pathways is often lost when individual signaling events are monitored in isolation. As the development of biosensor technology continues to advance, it is becoming increasingly feasible to image multiple FP-based biosensors concurrently, permitting greater insights into the intricate coordination of intracellular signaling networks by enabling parallel monitoring of distinct signaling events within the same cell. In this review, we discuss several strategies for multiplexed imaging of FP-based biosensors, while also underscoring some of the challenges associated with these techniques and highlighting additional avenues that could lead to further improvements in parallel monitoring of intracellular signaling events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.