ArticleMethods (San Diego, Calif.)2012
From cells to chromatin: capturing snapshots of genome organization with 5C technology.
Article in Methods (San Diego, Calif.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed.
- Writing the Engram: Epigenetic Mechanisms of Memory Allocation.Journal of neurochemistry · 2025Review
- Hidden secrets of the cancer genome: unlocking the impact of non-coding mutations in gene regulatory elements.Cellular and molecular life sciences : CMLS · 2024Review
- Comparative study on chromatin loop callers using Hi-C data reveals their effectiveness.BMC bioinformatics · 2024Article
- Bacon: a comprehensive computational benchmarking framework for evaluating targeted chromatin conformation capture-specific methodologies.Genome biology · 2022Article
- Inferring Single-Cell 3D Chromosomal Structures Based on the Lennard-Jones Potential.International journal of molecular sciences · 2021Article
- Liquid chromatin Hi-C characterizes compartment-dependent chromatin interaction dynamics.Nature genetics · 2021Article
- Three-dimensional chromatin in infectious disease-A role for gene regulation and pathogenicity?PLoS pathogens · 2021Review
- Dynamic Chromatin Structure and Epigenetics Control the Fate of Malaria Parasites.Trends in genetics : TIG · 2021Review
- Computational approaches for inferring 3D conformations of chromatin from chromosome conformation capture data.Methods (San Diego, Calif.) · 2020Review
- Hi-C chromosome conformation capture sequencing of avian genomes using the BGISEQ-500 platform.GigaScience · 2020Article
- From Genes to Transcripts, a Tightly Regulated Journey inFrontiers in cellular and infection microbiology · 2020Review
- 2C-ChIP: measuring chromatin immunoprecipitation signal from defined genomic regions with deep sequencing.BMC genomics · 2019Article
- Article
- 5C-ID: Increased resolution Chromosome-Conformation-Capture-Carbon-Copy with in situ 3C and double alternating primer design.Methods (San Diego, Calif.) · 2018Article
- Novel insights into chromosomal conformations in cancer.Molecular cancer · 2017Review
- Towards quantitative analysis of gene regulation by enhancers.Epigenomics · 2017Review
- Hi-C 2.0: An optimized Hi-C procedure for high-resolution genome-wide mapping of chromosome conformation.Methods (San Diego, Calif.) · 2017Article
- Priming of transcriptional memory responses via the chromatin accessibility landscape in T cells.Scientific reports · 2017Article
- Reciprocal regulation of chromatin state and architecture by HOTAIRM1 contributes to temporal collinear HOXA gene activation.Nucleic acids research · 2017Article
- Chromosome conformation capture technologies and their impact in understanding genome function.Chromosoma · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
In eukaryotes, genome organization can be observed on many levels and at different scales. This organization is important not only to reduce chromosome length but also for the proper execution of various biological processes. High-resolution mapping of spatial chromatin structure was made possible by the development of the chromosome conformation capture (3C) technique. 3C uses chemical cross-linking followed by proximity-based ligation of fragmented DNA to capture frequently interacting chromatin segments in cell populations. Several 3C-related methods capable of higher chromosome conformation mapping throughput were reported afterwards. These techniques include the 3C-carbon copy (5C) approach, which offers the advantage of being highly quantitative and reproducible. We provide here an updated reference protocol for the production of 5C libraries analyzed by next-generation sequencing or onto microarrays. A procedure used to verify that 3C library templates bear the high quality required to produce superior 5C libraries is also described. We believe that this detailed protocol will help guide researchers in probing spatial genome organization and its role in various biological processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.