ArticleBMC microbiology2012
FungiQuant: a broad-coverage fungal quantitative real-time PCR assay.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
118 citing papers in PubMed.
- Nivolumab plus ipilimumab with or without live bacterial supplementation in metastatic renal cell carcinoma: a randomized phase 1 trial.Nature medicine · 2022Trial
- The Candida albicans exotoxin candidalysin promotes alcohol-associated liver disease.Journal of hepatology · 2020Trial
- A Direct DNA Extraction Workflow for Metabarcoding Fungal Bioaerosols From Adhesive Samplers.Environmental microbiology · 2026Article
- Testing for Bacteria and Fungi in Cells and Ancillary Reagents-Probe-Based Quantitative PCR Assays.Cell proliferation · 2026Article
- Cathepsin B, Airway Pathogens, and Inflammation in the Lower Airways of Children With Cystic Fibrosis.Pediatric pulmonology · 2026Article
- Comparing rapid molecular and culture methods for detecting fungal contamination in healthcare environments.Infection control and hospital epidemiology · 2026Observational
- Enrichment of a mixed microbial culture for PHA production with used cooking oil as substrate.Bioprocess and biosystems engineering · 2026Article
- M.globosa promotes lung cancer progression and M2 macrophage polarization through oxidative phosphorylation.NPJ precision oncology · 2026Article
- Floral microbes provisioned by Osmia lignaria establish in larval food stores, but do not affect bee development or survival.FEMS microbiology ecology · 2026Article
- Control of Brominated and Iodinated DBPs within a Drinking Water Distribution System with Granular Activated Carbon and Biological Filtration.Environmental science & technology · 2026Article
- Gut-lung microbial dynamics with lumacaftor/ivacaftor in children with cystic fibrosis: a prospective multicenter study.Pediatric research · 2026Article
- Deep subsurface organic-rich shale supports abundant, diverse, and novel fungi.The ISME journal · 2026Article
- Salivary mycobial profiles of oral cancer patients - a cohort study.Journal of oral microbiology · 2026Article
- Dye-based qPCR assays for the separate quantification of total bacterial, fungal, arthropod, and plant DNA.PloS one · 2026Article
- Quality control strategy for differentiating induced pluripotent stem cells into pancreatic β-cells before transplantation.Regenerative medicine · 2026Review
- A propolis-rich hive environment affects redox gene expression and gut microbiota at the individual and social level in honey bees.FEMS microbes · 2026Article
- Microbial load and composition of intrauterine device string correlates with symptomatic removals in parous women.Contraception and reproductive medicine · 2025Article
- Real-time PCR assay design and validation for Prevotella bivia, Peptostreptococcus anaerobius, and Dialister micraerophilus, bacteria associated with increased HIV susceptibility.Scientific reports · 2025Article
- Quantification of airborne fungal antigens by ELISA and comparison to molecular biological and classical methods.Applied and environmental microbiology · 2025Article
- Increased fungal burden in the gastrointestinal tract of brain-dead organ donors.Microbiology spectrum · 2025Article
58 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
backgroundFungal load quantification is a critical component of fungal community analyses. Limitation of current approaches for quantifying the fungal component in the human microbiome suggests the need for new broad-coverage techniques.
methodsWe analyzed 2,085 18S rRNA gene sequences from the SILVA database for assay design. We generated and quantified plasmid standards using a qPCR-based approach. We evaluated assay coverage against 4,968 sequences and performed assay validation following the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines.
resultsWe designed FungiQuant, a TaqMan® qPCR assay targeting a 351 bp region in the fungal 18S rRNA gene. Our in silico analysis showed that FungiQuant is a perfect sequence match to 90.0% of the 2,617 fungal species analyzed. We showed that FungiQuant's is 100% sensitive and its amplification efficiencies ranged from 76.3% to 114.5%, with r(2)-values of >0.99 against the 69 fungal species tested. Additionally, FungiQuant inter- and intra-run coefficients of variance ranged from <10% and <20%, respectively. We further showed that FungiQuant has a limit of quantification 25 copies and a limit of detection at 5 copies. Lastly, by comparing results from human-only background DNA with low-level fungal DNA, we showed that amplification in two or three of a FungiQuant performed in triplicate is statistically significant for true positive fungal detection.
conclusionsFungiQuant has comprehensive coverage against diverse fungi and is a robust quantification and detection tool for delineating between true fungal detection and non-target human DNA.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.