ArticleBreeding science2012
Expressed sequence tags from organ-specific cDNA libraries of tea (Camellia sinensis) and polymorphisms and transferability of EST-SSRs across Camellia species.
Article in Breeding science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 25 citations in OpenAlex.
- Chromosome-Scale Genome Assembly and Characterization of Top-Quality Japanese Green Tea Cultivar 'Seimei'.Plant & cell physiology · 2024Article
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- Genome Survey and SSR Analysis ofGenetics research · 2022Article
- Convergent Biochemical Pathways for Xanthine Alkaloid Production in Plants Evolved from Ancestral Enzymes with Different Catalytic Properties.Molecular biology and evolution · 2021Article
- A new DNA marker CafLess-TCS1 for selection of caffeine-less tea plants.Breeding science · 2019Article
- Comparative genomics analysis reveals gene family expansion and changes of expression patterns associated with natural adaptations of flowering time and secondary metabolism in yellow Camellia.Functional & integrative genomics · 2018Article
- Investigation of Differences in Fertility among Progenies from Self-Pollinated Chrysanthemum.International journal of molecular sciences · 2018Article
- Biotechnological advances in tea (Camellia sinensis [L.] O. Kuntze): a review.Plant cell reports · 2016Review
- Article
- Next-generation sequencing reveals differentially amplified tandem repeats as a major genome component of Northern Europe's oldest Camellia japonica.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Article
- Varietal identification of tea (Camellia sinensis) using nanofluidic array of single nucleotide polymorphism (SNP) markers.Horticulture research · 2014Article
- Construction of a SSR-based genetic map and identification of QTLs for catechins content in tea plant (Camellia sinensis).PloS one · 2014Article
- Resilient plant-bird interactions in a volcanic island ecosystem: pollination of Japanese Camellia mediated by the Japanese White-eye.PloS one · 2013Article
- Construction of a high-density reference linkage map of tea (Camellia sinensis).Breeding science · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tea is one of the most popular beverages in the world and the tea plant, Camellia sinensis (L.) O. Kuntze, is an important crop in many countries. To increase the amount of genomic information available for C. sinensis, we constructed seven cDNA libraries from various organs and used these to generate expressed sequence tags (ESTs). A total of 17,458 ESTs were generated and assembled into 5,262 unigenes. About 50% of the unigenes were assigned annotations by Gene Ontology. Some were homologous to genes involved in important biological processes, such as nitrogen assimilation, aluminum response, and biosynthesis of caffeine and catechins. Digital northern analysis showed that 67 unigenes were expressed differentially among the seven organs. Simple sequence repeat (SSR) motif searches among the unigenes identified 1,835 unigenes (34.9%) harboring SSR motifs of more than six repeat units. A subset of 100 EST-SSR primer sets was tested for amplification and polymorphism in 16 tea accessions. Seventy-one primer sets successfully amplified EST-SSRs and 70 EST-SSR loci were polymorphic. Furthermore, these 70 EST-SSR markers were transferable to 14 other Camellia species. The ESTs and EST-SSR markers will enhance the study of important traits and the molecular genetics of tea plants and other Camellia species.
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