ArticleThe Plant cell2012
Crystal structure of an indole-3-acetic acid amido synthetase from grapevine involved in auxin homeostasis.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 101 citations in OpenAlex.
- GH3 phylogenetic subgroups define divergent routes of auxin inactivation via aspartate and glutamine conjugation.Plant physiology · 2026Article
- Integrated transcriptome-metabolome analyses reveal regulatory networks underlying soluble solids accumulation in Capsicum chinense fruits.The Plant journal : for cell and molecular biology · 2026Article
- The response of Petunia × atkinsiana 'Pegasus Special Burgundy Bicolor' to mechanical stress encompassing morphological changes as well as physiological and molecular factors.Scientific reports · 2025Article
- Transcriptome Reveals the Regulation of Exogenous Auxin Inducing Rooting of Non-Rooting Callus of Tea Cuttings.International journal of molecular sciences · 2024Article
- An auxin research odyssey: 1989-2023.The Plant cell · 2024Review
- The Roles of GRETCHEN HAGEN3 (GH3)-Dependent Auxin Conjugation in the Regulation of Plant Development and Stress Adaptation.Plants (Basel, Switzerland) · 2023Review
- Recent advances in auxin biosynthesis and homeostasis.3 Biotech · 2023Review
- Analysis of oxidase activity and transcriptomic changes related to cutting propagation of hybrid larch.Scientific reports · 2023Article
- Combined Transcriptomic and Metabolomic Analysis Reveals Insights into Resistance of ArabidopsisPlants (Basel, Switzerland) · 2022Article
- Integrated transcriptome and hormonal analysis of naphthalene acetic acid-induced adventitious root formation of tea cuttings (Camellia sinensis).BMC plant biology · 2022Article
- Alkaline Stress Induces Different Physiological, Hormonal and Gene Expression Responses in Diploid and Autotetraploid Rice.International journal of molecular sciences · 2022Article
- Genome-wide association study of the candidate genes for grape berry shape-related traits.BMC plant biology · 2022Article
- Auxin Treatment Enhances Anthocyanin Production in the Non-Climacteric Sweet Cherry (International journal of molecular sciences · 2021Article
- Genome-Wide Analysis, Modeling, and Identification of Amino Acid Binding Motifs Suggest the Involvement ofPlants (Basel, Switzerland) · 2021Article
- Chemical Biology in Auxin Research.Cold Spring Harbor perspectives in biology · 2021Review
- Genome-wide identification of GH3 genes in Brassica oleracea and identification of a promoter region for anther-specific expression of a GH3 gene.BMC genomics · 2021Article
- A novel insight into nitrogen and auxin signaling in lateral root formation in tea plant [Camellia sinensis (L.) O. Kuntze].BMC plant biology · 2020Article
- Brassicaceae-specific Gretchen Hagen 3 acyl acid amido synthetases conjugate amino acids to chorismate, a precursor of aromatic amino acids and salicylic acid.The Journal of biological chemistry · 2019Article
- Genome-wide analysis, transcription factor network approach and gene expression profile of GH3 genes over early somatic embryogenesis in Coffea spp.BMC genomics · 2019Article
- Auxin-Induced Adventitious Root Formation in Nodal Cuttings ofInternational journal of molecular sciences · 2019Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Auxins are important for plant growth and development, including the control of fruit ripening. Conjugation to amino acids by indole-3-acetic acid (IAA)-amido synthetases is an important part of auxin homeostasis. The structure of the auxin-conjugating Gretchen Hagen3-1 (GH3-1) enzyme from grapevine (Vitis vinifera), in complex with an inhibitor (adenosine-5'-[2-(1H-indol-3-yl)ethyl]phosphate), is presented. Comparison with a previously published benzoate-conjugating enzyme from Arabidopsis thaliana indicates that grapevine GH3-1 has a highly similar domain structure and also undergoes a large conformational change during catalysis. Mutational analyses and structural comparisons with other proteins have identified residues likely to be involved in acyl group, amino acid, and ATP substrate binding. Vv GH3-1 is a monomer in solution and requires magnesium ions solely for the adenlyation reaction. Modeling of IAA and two synthetic auxins, benzothiazole-2-oxyacetic acid (BTOA) and 1-naphthaleneacetic acid (NAA), into the active site indicates that NAA and BTOA are likely to be poor substrates for this enzyme, confirming previous enzyme kinetic studies. This suggests a reason for the increased effectiveness of NAA and BTOA as auxins in planta and provides a tool for designing new and effective auxins.
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