ArticleAmerican journal of physiology. Renal physiology2013
Response of the mitochondrial proteome of rat renal proximal convoluted tubules to chronic metabolic acidosis.
Article in American journal of physiology. Renal physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Article
- From Molecules to Mechanisms: Functional Proteomics and Its Application to Renal Tubule Physiology.Physiological reviews · 2018Review
- Proximal tubule-specific glutamine synthetase deletion alters basal and acidosis-stimulated ammonia metabolism.American journal of physiology. Renal physiology · 2016Article
- Mitochondrial proteomes of porcine kidney cortex and medulla: foundation for translational proteomics.Clinical and experimental nephrology · 2016Article
- Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis.BioMed research international · 2016Article
- Effects of cyclin-dependent kinase inhibitor Purvalanol B application on protein expression and developmental progression in intra-erythrocytic Plasmodium falciparum parasites.Malaria journal · 2015Article
- Simultaneously targeting tissue transglutaminase and kidney type glutaminase sensitizes cancer cells to acid toxicity and offers new opportunities for therapeutic intervention.Molecular pharmaceutics · 2015Article
- Proximal tubule function and response to acidosis.Clinical journal of the American Society of Nephrology : CJASN · 2014Review
- pH-responsive, gluconeogenic renal epithelial LLC-PK1-FBPase+cells: a versatile in vitro model to study renal proximal tubule metabolism and function.American journal of physiology. Renal physiology · 2014Review
- Proteomic Profiling of Sugar Beet (Proteomes · 2014Article
- Proteomic profiling and pathway analysis of the response of rat renal proximal convoluted tubules to metabolic acidosis.American journal of physiology. Renal physiology · 2013Article
- Proteomic profiling of the mitochondrial inner membrane of rat renal proximal convoluted tubules.Proteomics · 2013Article
- Mass spectrometry contamination from Tinuvin 770, a common additive in laboratory plastics.Journal of biomolecular techniques : JBT · 2013Article
- Improved detection of quantitative differences using a combination of spectral counting and MS/MS total ion current.Journal of proteome research · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Metabolic acidosis is a common clinical condition that is caused by a decrease in blood pH and bicarbonate concentration. Increased extraction and mitochondrial catabolism of plasma glutamine within the renal proximal convoluted tubule generates ammonium and bicarbonate ions that facilitate the excretion of acid and partially restore acid-base balance. Previous studies identified only a few mitochondrial proteins, including two key enzymes of glutamine metabolism, which are increased during chronic acidosis. A workflow was developed to characterize the mitochondrial proteome of the proximal convoluted tubule. Based upon the increase in specific activity of cytochrome c oxidase, the isolated mitochondria were enriched eightfold. Two-dimensional liquid chromatography coupled with mass spectrometry was utilized to compare mitochondrial-enriched samples from control and chronic acidotic rats. Proteomic analysis identified 901 proteins in the control and acidotic samples. Further analysis identified 37 peptides that contain an N-ε-acetyl-lysine; of these, 22 are novel sites. Spectral counting analysis revealed 33 proteins that are significantly altered in abundance in response to chronic metabolic acidosis. Western blot analysis was performed to validate the calculated changes in abundance. Thus the current study represents the first comprehensive analysis of the mitochondrial proteome of the rat renal proximal convoluted tubule and its response to metabolic acidosis.
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