ArticleJournal of clinical microbiology2013
Public health value of next-generation DNA sequencing of enterohemorrhagic Escherichia coli isolates from an outbreak.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
- One day in Denmark: whole-genome sequence-based analysis of Escherichia coli isolates from clinical settings.The Journal of antimicrobial chemotherapy · 2025Article
- Genome Sequence Analysis and Characterization of Shiga Toxin 2 Production byFrontiers in cellular and infection microbiology · 2022Article
- Whole-genome analyses of extended-spectrum or AmpC β-lactamase-producing Escherichia coli isolates from companion dogs in Japan.PloS one · 2021Article
- Assessing the genomic relatedness and evolutionary rates of persistent verotoxigenicMicrobial genomics · 2020Article
- Fishing in the Soup - Pathogen Detection in Food Safety Using Metabarcoding and Metagenomic Sequencing.Frontiers in microbiology · 2019Article
- Impact of whole genome sequencing on the investigation of food-borne outbreaks of Shiga toxin-producingEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2019Article
- Evaluation of whole-genome sequencing for outbreak detection of Verotoxigenic Escherichia coli O157:H7 from the Canadian perspective.BMC genomics · 2018Article
- Validation of Whole-Genome Sequencing for Identification and Characterization of Shiga Toxin-Producing Escherichia coli To Produce Standardized Data To Enable Data Sharing.Journal of clinical microbiology · 2018Article
- Interpreting Whole-Genome Sequence Analyses of Foodborne Bacteria for Regulatory Applications and Outbreak Investigations.Frontiers in microbiology · 2018Article
- 16S rRNA gene sequencing on a benchtop sequencer: accuracy for identification of clinically important bacteria.Journal of applied microbiology · 2017Article
- Real-time genomic investigation underlying the public health response to a Shiga toxin-producing Escherichia coli O26:H11 outbreak in a nursery.Epidemiology and infection · 2017Article
- Single-Nucleotide Polymorphisms in the Whole-Genome Sequence Data of Shiga Toxin-Producing Escherichia coli O157:H7/H- Strains by Cultivation.Current microbiology · 2017Article
- High Throughput Sequencing for Detection of Foodborne Pathogens.Frontiers in microbiology · 2017Review
- A Geographically Widespread Outbreak Investigation and Development of a Rapid Screening Method Using Whole Genome Sequences of EnterohemorrhagicFrontiers in microbiology · 2017Article
- Navigating Microbiological Food Safety in the Era of Whole-Genome Sequencing.Clinical microbiology reviews · 2016Review
- Trends in ExPEC serogroups in the UK and their significance.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Whole genome sequencing of Salmonella Typhimurium illuminates distinct outbreaks caused by an endemic multi-locus variable number tandem repeat analysis type in Australia, 2014.BMC microbiology · 2016Article
- An outbreak of Shiga toxin-producing Escherichia coli serogroup O157 linked to a lamb-feeding event.Epidemiology and infection · 2016Article
- FDA Escherichia coli Identification (FDA-ECID) Microarray: a Pangenome Molecular Toolbox for Serotyping, Virulence Profiling, Molecular Epidemiology, and Phylogeny.Applied and environmental microbiology · 2016Article
- Whole Genome Sequencing for Genomics-Guided Investigations of Escherichia coli O157:H7 Outbreaks.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
In 2009, an outbreak of enterohemorrhagic Escherichia coli (EHEC) on an open farm infected 93 persons, and approximately 22% of these individuals developed hemolytic-uremic syndrome (HUS). Genome sequencing was used to investigate outbreak-derived animal and human EHEC isolates. Phylogeny based on the whole-genome sequence was used to place outbreak isolates in the context of the overall E. coli species and the O157:H7 sequence type 11 (ST11) subgroup. Four informative single nucleotide polymorphisms (SNPs) were identified and used to design an assay to type 122 other outbreak isolates. The SNP phylogeny demonstrated that the outbreak strain was from a lineage distinct from previously reported O157:H7 ST11 EHEC and was not a member of the hypervirulent clade 8. The strain harbored determinants for two Stx2 verotoxins and other putative virulence factors. When linked to the epidemiological information, the sequence data indicate that gross contamination of a single outbreak strain occurred across the farm prior to the first clinical report of HUS. The most likely explanation for these results is that a single successful strain of EHEC spread from a single introduction through the farm by clonal expansion and that contamination of the environment (including the possible colonization of several animals) led ultimately to human cases.
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