ArticleThe Journal of biological chemistry2012
Mediator phosphorylation prevents stress response transcription during non-stress conditions.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Review
- Mediator dynamics during heat shock in budding yeast.Genome research · 2022Article
- Cdk8 Kinase Module: A Mediator of Life and Death Decisions in Times of Stress.Microorganisms · 2021Review
- The Polymorphic PolyQ Tail Protein of the Mediator Complex, Med15, Regulates the Variable Response to Diverse Stresses.International journal of molecular sciences · 2020Article
- Role of Mediator in virulence and antifungal drug resistance in pathogenic fungi.Current genetics · 2019Review
- Border collies of the genome: domestication of an autonomous retrovirus-like transposon.Current genetics · 2019Review
- Transcription regulation by the Mediator complex.Nature reviews. Molecular cell biology · 2018Review
- Control of Muscle Metabolism by the Mediator Complex.Cold Spring Harbor perspectives in medicine · 2018Review
- The Mediator co-activator complex regulates Ty1 retromobility by controlling the balance between Ty1i and Ty1 promoters.PLoS genetics · 2018Article
- Med15B Regulates Acid Stress Response and Tolerance in Candida glabrata by Altering Membrane Lipid Composition.Applied and environmental microbiology · 2017Article
- Article
- Combined Antibody/Lectin Enrichment Identifies Extensive Changes in the O-GlcNAc Sub-proteome upon Oxidative Stress.Journal of proteome research · 2016Article
- Evolution of disorder in Mediator complex and its functional relevance.Nucleic acids research · 2016Article
- Analysis of the Candida albicans Phosphoproteome.Eukaryotic cell · 2015Article
- Muscle as a "mediator" of systemic metabolism.Cell metabolism · 2015Review
- Telomeric ORFS in Candida albicans: does mediator tail wag the yeast?PLoS pathogens · 2015Review
- Genome-wide association of mediator and RNA polymerase II in wild-type and mediator mutant yeast.Molecular and cellular biology · 2015Article
- Telomeric ORFs (TLOs) in Candida spp. Encode mediator subunits that regulate distinct virulence traits.PLoS genetics · 2014Article
- Mediator recruitment to heat shock genes requires dual Hsf1 activation domains and mediator tail subunits Med15 and Med16.The Journal of biological chemistry · 2013Article
- Conserved versatile master regulators in signalling pathways in response to stress in plants.AoB PLANTS · 2013Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The multiprotein complex Mediator is a coactivator of RNA polymerase (Pol) II transcription that is required for the regulated expression of protein-coding genes. Mediator serves as an end point of signaling pathways and regulates Pol II transcription, but the mechanisms it uses are not well understood. Here, we used mass spectrometry and dynamic transcriptome analysis to investigate a functional role of Mediator phosphorylation in gene expression. Affinity purification and mass spectrometry revealed that Mediator from the yeast Saccharomyces cerevisiae is phosphorylated at multiple sites of 17 of its 25 subunits. Mediator phosphorylation levels change upon an external stimulus set by exposure of cells to high salt concentrations. Phosphorylated sites in the Mediator tail subunit Med15 are required for suppression of stress-induced changes in gene expression under non-stress conditions. Thus dynamic and differential Mediator phosphorylation contributes to gene regulation in eukaryotic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.