ArticleBMC plant biology2012
A diploid wheat TILLING resource for wheat functional genomics.
Article in BMC plant biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- Wheat improvement through advances in single nucleotide polymorphism (SNP) detection and genotyping with a special emphasis on rust resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Integration of genetic and genomics resources in einkorn wheat enables precision mapping of important traits.Communications biology · 2023Article
- Evaluation of altered starch mutants and identification of candidate genes responsible for starch variation in wheat.BMC plant biology · 2023Article
- Boosting wheat functional genomics via an indexed EMS mutant library of KN9204.Plant communications · 2023Article
- Variations in Total Protein and Amino Acids in the Sequenced Sorghum Mutant Library.Plants (Basel, Switzerland) · 2023Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Triple null mutations in starch synthase SSIIa gene homoeologs lead to high amylose and resistant starch in hexaploid wheat.BMC plant biology · 2021Article
- Bymovirus-induced yellow mosaic diseases in barley and wheat: viruses, genetic resistances and functional aspects.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Functional mutation allele mining of plant architecture and yield-related agronomic traits and characterization of their effects in wheat.BMC genetics · 2019Article
- Phenotypic and molecular characterization of Hessian fly resistance in diploid wheat, Aegilops tauschii.BMC plant biology · 2019Article
- Rapid gene cloning in cereals.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
- Genetic transformation of einkorn (Triticum monococcum L. ssp. monococcum L.), a diploid cultivated wheat species.BMC biotechnology · 2018Article
- TILL-D: AnFrontiers in plant science · 2018Article
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- Identification of induced mutations in hexaploid wheat genome using exome capture assay.PloS one · 2018Article
- Translational genomics of grain size regulation in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Review
- mlo-based powdery mildew resistance in hexaploid bread wheat generated by a non-transgenic TILLING approach.Plant biotechnology journal · 2017Article
- Induced Mutagenesis inFrontiers in plant science · 2017Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTriticum monococcum L., an A genome diploid einkorn wheat, was the first domesticated crop. As a diploid, it is attractive genetic model for the study of gene structure and function of wheat-specific traits. Diploid wheat is currently not amenable to reverse genetics approaches such as insertion mutagenesis and post-transcriptional gene silencing strategies. However, TILLING offers a powerful functional genetics approach for wheat gene analysis.
resultsWe developed a TILLING population of 1,532 M2 families using EMS as a mutagen. A total of 67 mutants were obtained for the four genes studied. Waxy gene mutation frequencies are known to be 1/17.6 - 34.4 kb DNA in polyploid wheat TILLING populations. The T. monococcum diploid wheat TILLING population had a mutation frequency of 1/90 kb for the same gene. Lignin biosynthesis pathway genes- COMT1, HCT2, and 4CL1 had mutation frequencies of 1/86 kb, 1/92 kb and 1/100 kb, respectively. The overall mutation frequency of the diploid wheat TILLING population was 1/92 kb.
conclusionThe mutation frequency of a diploid wheat TILLING population was found to be higher than that reported for other diploid grasses. The rate, however, is lower than tetraploid and hexaploid wheat TILLING populations because of the higher tolerance of polyploids to mutations. Unlike polyploid wheat, most mutants in diploid wheat have a phenotype amenable to forward and reverse genetic analysis and establish diploid wheat as an attractive model to study gene function in wheat. We estimate that a TILLING population of 5, 520 will be needed to get a non-sense mutation for every wheat gene of interest with 95% probability.
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