ArticleMicrobial cell factories2012
Design and testing of a synthetic biology framework for genetic engineering of Corynebacterium glutamicum.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
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- Bacteriophages target membrane-anchored glycopolymers to promote host cell lysis and progeny release.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
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- Cryo-EM structure and polar assembly of the PS2 S-layer ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Intercepting a Mycobacterial Biosynthetic Pathway with Covalent Labeling.Journal of the American Chemical Society · 2025Article
- Differential substrate preferences IN ACTINOBACTERIAL protein O-MANNOSYLTRANSFERASES and alteration of protein-O-MANNOSYLATION by choice of secretion pathway.Glycobiology · 2025Article
- The conserved σD envelope stress response monitors multiple aspects of envelope integrity in corynebacteria.PLoS genetics · 2024Article
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- Construction of an ultra-strong PtacM promoter via engineering the core-element spacer and 5' untranslated region for versatile applications inBiotechnology notes (Amsterdam, Netherlands) · 2022Article
- Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum.Nature communications · 2020Article
- Article
- Ribosomal binding site sequences and promoters for expressing glutamate decarboxylase and producing γ-aminobutyrate in Corynebacterium glutamicum.AMB Express · 2018Article
- Mu-driven transposition of recombinant mini-Mu unit DNA in the Corynebacterium glutamicum chromosome.Applied microbiology and biotechnology · 2018Article
- CamOptimus: a tool for exploiting complex adaptive evolution to optimize experiments and processes in biotechnology.Microbiology (Reading, England) · 2017Article
- Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid.Microbial cell factories · 2016Article
- Membrane anchoring stabilizes and favors secretion of New Delhi metallo-β-lactamase.Nature chemical biology · 2016Article
- Synthetic promoter design for new microbial chassis.Biochemical Society transactions · 2016Review
- Ribosome binding site libraries and pathway modules for shikimic acid synthesis with Corynebacterium glutamicum.Microbial cell factories · 2015Article
- A novel locus for mycelial aggregation forms a gateway to improved Streptomyces cell factories.Microbial cell factories · 2015Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSynthetic biology approaches can make a significant contribution to the advance of metabolic engineering by reducing the development time of recombinant organisms. However, most of synthetic biology tools have been developed for Escherichia coli. Here we provide a platform for rapid engineering of C. glutamicum, a microorganism of great industrial interest. This bacteria, used for decades for the fermentative production of amino acids, has recently been developed as a host for the production of several economically important compounds including metabolites and recombinant proteins because of its higher capacity of secretion compared to traditional bacterial hosts like E. coli. Thus, the development of modern molecular platforms may significantly contribute to establish C. glutamicum as a robust and versatile microbial factory.
resultsA plasmid based platform named pTGR was created where all the genetic components are flanked by unique restriction sites to both facilitate the evaluation of regulatory sequences and the assembly of constructs for the expression of multiple genes. The approach was validated by using reporter genes to test promoters, ribosome binding sites, and for the assembly of dual gene operons and gene clusters containing two transcriptional units. Combinatorial assembly of promoter (tac, cspB and sod) and RBS (lacZ, cspB and sod) elements with different strengths conferred clear differential gene expression of two reporter genes, eGFP and mCherry, thus allowing transcriptional "fine-tuning"of multiple genes. In addition, the platform allowed the rapid assembly of operons and genes clusters for co-expression of heterologous genes, a feature that may assist metabolic pathway engineering.
conclusionsWe anticipate that the pTGR platform will contribute to explore the potential of novel parts to regulate gene expression, and to facilitate the assembly of genetic circuits for metabolic engineering of C. glutamicum. The standardization provided by this approach may provide a means to improve the productivity of biosynthetic pathways in microbial factories for the production of novel compounds.
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