ArticlePloS one2012
Identification of the functional domains of the telomere protein Rap1 in Schizosaccharomyces pombe.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 37 citations in OpenAlex.
- Combinatorial Analyses of Pre-mRNA Splicing-Defective Mutants Reveal Differential Quantitative Requirements for Shelterin in Distinct Telomere Functions.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
- Characterizing Cellular Physiological States with Three-Dimensional Shape Descriptors for Cell Membranes.Membranes · 2024Article
- Unwrap RAP1's Mystery at Kinetoplastid Telomeres.Biomolecules · 2024Review
- Nuclear genome organization in fungi: from gene folding to Rabl chromosomes.FEMS microbiology reviews · 2023Article
- Roles of Specialized Chromatin and DNA Structures at Subtelomeres inBiomolecules · 2023Review
- A proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells.Nucleic acids research · 2022Article
- Rap1 prevents fusions between long telomeres in fission yeast.The EMBO journal · 2022Article
- Splicing of branchpoint-distant exons is promoted by Cactin, Tls1 and the ubiquitin-fold-activated Sde2.Nucleic acids research · 2022Article
- Step-by-Step Evolution of Telomeres: Lessons from Yeasts.Genome biology and evolution · 2021Article
- Complete sequences of Schizosaccharomyces pombe subtelomeres reveal multiple patterns of genome variation.Nature communications · 2021Article
- Trypanosoma brucei RAP1 Has Essential Functional Domains That Are Required for Different Protein Interactions.mSphere · 2020Article
- Telomere-binding proteins Taz1 and Rap1 regulate DSB repair and suppress gross chromosomal rearrangements in fission yeast.PLoS genetics · 2019Article
- Casein kinase 2 regulates telomere protein complex formation through Rap1 phosphorylation.Nucleic acids research · 2019Article
- Telomere DNA length-dependent regulation of DNA replication timing at internal late replication origins.Scientific reports · 2019Article
- Construction of MC1R and ASIP Eukaryotic Expression Vector and its Regulation of Plumage Color in Japanese Quail (The journal of poultry science · 2019Article
- Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast.Nucleic acids research · 2019Article
- Structure of the fission yeast S. pombe telomeric Tpz1-Poz1-Rap1 complex.Cell research · 2017Article
- Structural Basis for Shelterin Bridge Assembly.Molecular cell · 2017Article
- A Matter of Scale and Dimensions: Chromatin of Chromosome Landmarks in the Fungi.Microbiology spectrum · 2017Review
- Timeless protection of telomeres.Current genetics · 2016Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The telomere at the end of a linear chromosome plays crucial roles in genome stability. In the fission yeast Schizosaccharomyces pombe, the Rap1 protein, one of the central players at the telomeres, associates with multiple proteins to regulate various telomere functions, such as the maintenance of telomere DNA length, telomere end protection, maintenance of telomere heterochromatin, and telomere clustering in meiosis. The molecular bases of the interactions between Rap1 and its partners, however, remain largely unknown. Here, we describe the identification of the interaction domains of Rap1 with its partners. The Bqt1/Bqt2 complex, which is required for normal meiotic progression, Poz1, which is required for telomere length control, and Taz1, which is required for the recruitment of Rap1 to telomeres, bind to distinct domains in the C-terminal half of Rap1. Intriguingly, analyses of a series of deletion mutants for rap1(+) have revealed that the long N-terminal region (1-456 a.a. [amino acids]) of Rap1 (full length: 693 a.a.) is not required for telomere DNA length control, telomere end protection, and telomere gene silencing, whereas the C-terminal region (457-693 a.a.) containing Poz1- and Taz1-binding domains plays important roles in those functions. Furthermore, the Bqt1/Bqt2- and Taz1-binding domains are essential for normal spore formation after meiosis. Our results suggest that the C-terminal half of Rap1 is critical for the primary telomere functions, whereas the N-terminal region containing the BRCT (BRCA1 C-terminus) and Myb domains, which are evolutionally conserved among the Rap1 family proteins, does not play a major role at the telomeres.
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