ArticlePloS one2012
Preferential release of newly synthesized insulin assessed by a multi-label reporter system using pancreatic β-cell line MIN6.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Cargo receptor Surf4 regulates endoplasmic reticulum export of proinsulin in pancreatic β-cells.Communications biology · 2022Article
- Isolation and Proteomics of the Insulin Secretory Granule.Metabolites · 2021Review
- Article
- Temporal Transcriptome Analysis Reveals Dynamic Gene Expression Patterns Driving β-Cell Maturation.Frontiers in cell and developmental biology · 2021Article
- A fluorescent timer reporter enables sorting of insulin secretory granules by age.The Journal of biological chemistry · 2020Article
- The pseudophosphatase phogrin enables glucose-stimulated insulin signaling in pancreatic β cells.The Journal of biological chemistry · 2018Article
- Lipoxygenase-mediated generation of lipid peroxides enhances ferroptosis induced by erastin and RSL3.Cancer science · 2017Article
- A stable isotope method for in vivo assessment of human insulin synthesis and secretion.Acta diabetologica · 2016Article
- Oxygenation and function of endocrine bioartificial pancreatic tissue constructs under flow for preclinical optimization.Journal of tissue engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Newly synthesized hormones have been suggested to be preferentially secreted by various neuroendocrine cells. This observation indicates that there is a distinct population of secretory granules containing new and old hormones. Recent development of fluorescent timer proteins used in bovine adrenal chromaffin cells revealed that secretory vesicles segregate into distinct age-dependent populations. Here, we verify the preferential release of newly synthesized insulin in the pancreatic β-cell line, MIN6, using a combination of multi-labeling reporter systems with both fluorescent and biochemical procedures. This system allows hormones or granules of any age to be labeled, in contrast to the timer proteins, which require fluorescence shift time. Pulse-chase labeling with different color probes distinguishes insulin secretory granules by age, with younger granules having a predominantly intracellular localization rather than at the cell periphery.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.