ArticlePloS one2012
Molecular cloning, expression and molecular modeling of chemosensory protein from Spodoptera litura and its binding properties with Rhodojaponin III.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Identification of Potential Gene Targets for Suppressing Oviposition inInternational journal of molecular sciences · 2023Article
- Article
- Odorant-Binding Proteins and Chemosensory Proteins inInternational journal of molecular sciences · 2023Article
- The Antennal Sensilla and Expression Patterns of Olfactory Genes in the Lower Termite Reticulitermes aculabialis (Isoptera: Rhinotermitidae).Journal of insect science (Online) · 2022Article
- The Crystal Structure of theInsects · 2021Article
- Article
- Molecular Modeling of Chemosensory Protein 3 fromInternational journal of molecular sciences · 2020Article
- Molecular characterization and functional differentiation of three pheromone-binding proteins from Tryporyza intacta.Scientific reports · 2018Article
- A chemosensory protein MsepCSP5 involved in chemoreception of oriental armywormInternational journal of biological sciences · 2018Article
- Functional Analysis of the Chemosensory Protein MsepCSP8 From the Oriental ArmywormFrontiers in physiology · 2018Article
- Effects of Pyriproxyfen on Female Reproduction in the Common Cutworm, Spodoptera litura (F.) (Lepidoptera: Noctuidae).PloS one · 2015Article
- Molecular cloning, modeling and differential expression of a gene encoding a silent information regulator-like protein from Sporothrix schenckii.International journal of molecular medicine · 2014Article
- Involvement of a specific chemosensory protein from Bactrocera dorsalis in perceiving host plant volatiles.Journal of chemical ecology · 2014Article
- Article
- BdorCSP2 is important for antifeed and oviposition-deterring activities induced by Rhodojaponin-III against Bactrocera dorsalis.PloS one · 2013Article
- Article
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7 authors.
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Abstract
Insects stimulate specific behaviors by the correct recognition of the chemicals in the external environment. Rhodojaponin III is a botanical grayanoid diterpenid oviposition deterrent isolated from Rhododendron molle. In this study we aimed to determine whether the CSPs involved in the recognition of Rhodojaponin III. A full-length cDNA encoding chemosensory protein was isolated from the antennae of Spodoptera litura Fabricius (CSPSlit, GenBank Accession No. DQ007458). The full-length cDNA of NlFoxA is 1789 bp and has an open reading frame (ORF) of 473 bp, encoding a protein of 126 amino acids, Northern blot analysis revealed that CSPSlit mRNA was mainly expressed in the antennae, legs, wings and female abdomens. A three-dimensional model of CSPSlit was constructed using homology modeling method, and its reliability was evaluated. The active site of CSPSlit was calculated using CDOCKER program indicated that the Tyr24, Ile45, Leu49, Thr64, Leu68, Trp79 and Leu82 were responsible ligand-binding active site on identifying Rhodojaponin III in the CSPSlit. The recombinant CSPSlit protein was expressed in Escherichia coli and purified using single-step Ni-NTA affinity chromatography. Fluorescence emission spectra revealed that the CSPSlit protein had significant affinity to rhodojaponin III. These results mean that CSPSlit is critical for insects identify the Rhodojaponin III.
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