ArticlePloS one2012
Dissecting systemic RNA interference in the red flour beetle Tribolium castaneum: parameters affecting the efficiency of RNAi.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers.
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Who cites it
97 citing papers in PubMed.
- Emerging experimental and bioinformatic approaches in RNA interference-based pest control research.Insect molecular biology · 2026Review
- RNAi-mediated knockdown of the poultry red mite cathepsin D-1 impacts haemoglobin digestion.Parasites & vectors · 2026Article
- Control Strategies of Plant Viruses Using Spray-Induced Gene Silencing.The plant pathology journal · 2026Review
- Smolstatin1, a unique cystatin-like stefin ofCurrent research in microbial sciences · 2026Article
- Distribution and efficacy of ingested dsRNA targeting tissue-specific genes in the Argentine ant, Linepithema humile.PloS one · 2026Article
- The functional of cathepsin L in the ontogenesis and reproductive regulation of the Asian corn borer, Ostrinia furnacalis.Scientific reports · 2025Article
- Targeting vacuolar ATPase in tobacco whitefly,Journal of biosciences · 2025Review
- SilencingInsects · 2024Article
- From Egg to Adult: A Developmental Table of the Ant Monomorium pharaonis.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Article
- RNA interference protocols for gene silencing in the spittlebug Philaenus spumarius, vector of Xylella fastidiosa.Scientific reports · 2024Article
- Review
- Using RNA interference targeting a nicotinic acetylcholine receptor subunit to counteract insecticide accommodation mechanisms: example of the β1 subunit in the imidacloprid-accommodated American cockroach,Journal of pesticide science · 2024Article
- Inefficient uptake of small interfering RNAs is responsible for their inability to trigger RNA interference in Colorado potato beetle cells.Archives of insect biochemistry and physiology · 2023Article
- Functional Divergence of theInsects · 2023Article
- Article
- The Study of Cell-Penetrating Peptides to Deliver dsRNA and siRNA by Feeding in the Desert Locust,Insects · 2023Article
- Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities.Functional & integrative genomics · 2023Review
- NHA1 is a cation/proton antiporter essential for the water-conserving functions of the rectal complex inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Transcriptomic exploration of the Coleopteran wings reveals insight into the evolution of novel structures associated with the beetle elytron.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2023Article
- Knockdown of the Sodium/Potassium ATPase Subunit Beta 2 Reduces Egg Production in the Dengue Vector,Insects · 2023Article
37 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The phenomenon of RNAi, in which the introduction of dsRNA into a cell triggers the destruction of the corresponding mRNA resulting in a gene silencing effect, is conserved across a wide array of plant and animal phyla. However, the mechanism by which the dsRNA enters a cell, allowing the RNAi effect to occur throughout a multicellular organism (systemic RNAi), has only been studied extensively in certain plants and the nematode Caenorhabditis elegans. In recent years, RNAi has become a popular reverse genetic technique for gene silencing in many organisms. Although many RNAi techniques in non-traditional model organisms rely on the systemic nature of RNAi, little has been done to analyze the parameters required to obtain a robust systemic RNAi response. The data provided here show that the concentration and length of dsRNA have profound effects on the efficacy of the RNAi response both in regard to initial efficiency and duration of the effect in Tribolium castaneum. In addition, our analyses using a series of short dsRNAs and chimeric dsRNA provide evidence that dsRNA cellular uptake (and not the RNAi response itself) is the major step affected by dsRNA size in Tribolium. We also demonstrate that competitive inhibition of dsRNA can occur when multiple dsRNAs are injected together, influencing the effectiveness of RNAi. These data provide specific information essential to the design and implementation of RNAi based studies, and may provide insight into the molecular basis of the systemic RNAi response in insects.
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